ResultsSandwich ELISASandwich ELISA performed with CD44 recombinant protein and CD44 monoclonal antibody (APC)ImmunofluorescenceImmunofluorescence staining performed on PC-3 cells using CD44 monoclonal antibody (APC) (10 ug/mL).
Recommend Usage:
Immunofluorescence (10 ug/mL)LiquidCell™ SuperSlide™ (6 uL per slide)
Storage Buffer:
In 1x PBS, pH 7.4
Storage Instruction:
Store at 2-8°C. Do not freeze.
Protocol:
Protocol Download
Amenable Platform:
LiquidCell™ negative enrichment cell isolation
Available Test:
16 assays
Regulatory Status:
GMP grade
Instrument Compatibility:
Ventana™ BenchMark Stainer
Datasheet:
Download
Applications
Immunofluorescence
Immunofluorescence staining performed on CTCs from the patient recoverd by SuperSlide™. CD44 was detected via red signal (APC).
The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis. [provided by RefSeq
Other Designations:
CD44 antigen,CD44 antigen (homing function and Indian blood group system),CDW44 antigen,GP90 lymphocyte homing/adhesion receptor,Hermes antigen,antigen gp90 homing receptor,cell adhesion molecule,cell surface glycoprotein CD44,chondroitin sulfate proteogl