ResultsSandwich ELISASandwich ELISA performed with CD45 recombinant protein and CD45 monoclonal antibody (Alexa 647)ImmunofluorescenceImmunofluorescence staining performed on Jurkat cells using CD45 monoclonal antibody (Alexa 647) (10 ug/mL).
Recommend Usage:
Immunofluorescence (10 ug/mL)LiquidCell™ SuperSlide™ (1.5 uL per slide)
Storage Buffer:
In 1x PBS, pH 7.4
Storage Instruction:
Store at 2-8°C. Do not freeze.
Protocol:
Protocol Download
Amenable Platform:
LiquidCell™ negative enrichment cell isolation
Available Test:
16 assays
Regulatory Status:
GMP grade
Instrument Compatibility:
Ventana™ BenchMark Stainer
Datasheet:
Download
Applications
Immunofluorescence
Immunofluorescence staining performed on WBCs recoverd by SuperSlide™. CD45 was detected via red signal (Alexa 647).
Application Image
Immunofluorescence
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Gene Information
Entrez GeneID:
5788
Gene Name:
PTPRC
Gene Alias:
B220,CD45,CD45R,GP180,LCA,LY5,T200
Gene Description:
protein tyrosine phosphatase, receptor type, C
Omim ID:
126200, 151460, 609532
Gene Ontology:
Hyperlink
Gene Summary:
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus belongs to receptor type PTP. This gene is specifically expressed in hematopoietic cells. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq
Other Designations:
CD45 antigen,T200 glycoprotein,T200 leukocyte common antigen,glycoprotein,leukocyte-common antigen,protein tyrosine phosphatase, receptor type, c polypeptide