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Millipore/DET-HP1000 | IHC Select® HRP Detection Set, (Blocking Reagent, Biotinylated linking Riagent and SA_HRP), Predi
  • Millipore/DET-HP1000 | IHC Select® HRP Detection Set, (Blocking Reagent, Biotinylated linking Riagent and SA_HRP), Predi

Millipore/DET-HP1000 | IHC Select® HRP Detection Set, (Blocking Reagent, Biotinylated linking Riagent and SA_HRP), Predi

价格: ¥6048.00 市场价: 10080.00
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货号: DET-HP1000
品牌: Millipore
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    • Description
      CatalogueNumberDET-HP1000
      Replaces2240
      BrandFamilyChemicon®
      TradeName
      • IHCSelect
      • Chemicon
      DescriptionIHCSelect®HRPDetectionSet,(BlockingReagent,BiotinylatedlinkingRiagentandSA_HRP),Prediluted
      OverviewIntendedUse

      TheIHCSelect®ImmunoperoxidaseSecondaryDetectionSystemisintendedforusewiththeCHEMICONIHCSelect®PredilutedPrimaryAntibodyreagents,whichcontainrabbitandmouseIgGforthequalitativeidentificationofantigensbylightmicroscopyinparaffin-embeddedtissues.TheIHCSelect®ImmunoperoxidaseSecondaryDetectionSystemmayalsobeusedwithotherrabbitormouseprimaryantibodies.

      GeneralPurposeReagents

      PrincipleofProcedure:

      TheIHCSelect®ImmunoperoxidaseSecondaryDetectionSystemusesbiotin-avidin-HRPcomplexedantibodiestodetectrabbitormouseIgGinthePrimaryAntibody.Theprimaryantibodybindstospecificantigenspresentinthespecimen.Anyexcessantibodyisremovedbywashing.Next,theSecondaryAntibodiesReagent(conjugatedtobiotin)isaddedandwillreactwiththeprimaryantibodypresentonthetissue.Unboundantibodyisremovedbywashing.ThesampleisthenbyincubatedwiththeStreptavidin-HRPsolution,whichbindstothebiotin-labeledsecondaryantibodypresentonthetissue.Unboundenzymeisremovedbywashing.Thechromogenicdevelopmentreagent,3,3"diaminobenzidine(DABSubstrate),isthenaddedandreactswiththeHRPattachedtotheHRP-streptavidin-biotin-antibodycomplex.TheHRPactivityonthechromogenicsubstrateresultsinthedepositofbrowntoblackinsolubleprecipitateatthoseantigenicsitescontainingthespecificepitopesrecognizedbytheprimaryantibody.Tissuesprocessedinavarietyoffixativesincludingneutralbufferedformalin,B-5,andBouin"smaybeused.

      SummaryandExplanation:

      Immunohistochemicaltechniqueshavebeeninusefortherecognitionofcellsintissuesectionsforatleast50years.Thefirstmethodreporteduseoffluorescentlabelsin19411.In1974,reportsofusingenzymaticlabels,suchashorserADIshperoxidase(HRP)inroutineH&Eparaffinsectionsappeared2,3.Themethodshavesincebecomethe"standardofcare"insurgicalpathologywhenclassicmethodsalonefailtoyieldadefinitivediagnosis4-9.

      Earlytechniqueswerebasedontheperoxidase-anti-peroxidase(PAP)reaction,whileimprovementsexploitedthestrongaffinityofavidinforbiotin10.ThistechniqueusesHRP-labeledstreptavidin.ThesecondaryantibodyisconjugatedwithbiotinandtheStrepavidin-HRPcomplexreactswiththebiotinonthesecondaryantibodies.Theresultingbiotin-avidin-HRPcomplexcanreactwiththeprimaryantibodyboundtothespecificepitopeofthetargetantigen.TheHRPenzymesofthestreptavidincomplexthencatalyzethesubstrate/chromogenreactiontoformacolouredreactionproduct(browntoblackwhenusingDABasthechromogen)attheantigensite.ABIOLOGicalstain(counterstain)isthenusedtovisualizethetissuestructure.
      MaterialsRequiredbutNotDelivered·PrimaryrabbitormouseIgGantibodies(suchasCHEMICONIHCSelect™PredilutedPrimaryAntibodies)

      ·WashBuffer

      ·Chromogen

      ·Counterstain

      ·EndogenousBlockingAgent

      ·Positiveandnegativetissuecontrolslides

      ·Positivelychargedmicroscopeslides

      ·Glasscoverslips(24x40mm,No.1)

      ·Distilledordeionizedwater

      ·Permanentmountingmedium,Eukitt*AmericanHistologyorequivalent

      ·3%laboratorygradehydrogenperoxide

      ·10.0%neutralbufferedformalin15

      ·Xylene,ACSgrade

      ·Ethanol,absolute(100%)

      ·Latex,rubberor100%nitrilegloves(xyleneresistant)

      ·Timer

      ·Fumehood

      ·LightmicroscopeequippedwithPlanAchromatorequivalentlenses

      ·60°Cdryingoven
      ProductInformation
      DetectionmethodChromogenic
      StorageandShippingInformation
      StorageConditionsWhenstoredat2-8°C,theIHCSelect™ImmunoperoxidaseSecondaryDetectionSystemisstableuptotheexpirationdateprintedonthekitlabel.Donotfreezeorexposetoelevatedtemperatures.Discardanyremainingreagentsaftertheexpirationdate.Donotstorekitcomponentsorperformstaininginstronglight,suchasdirectsunlight.

      Precautions:

      ·Pooling,substitutionoralterationofanyreagentmaycauseerroneousresults.

      *Donotmixreagentsfromdifferentlots.

      *Donotallowtheslidestodryatanytimeduringthestainingprocedure.

      *Handleallspecimensandmaterialscomingincontactwiththemaspotentiallyinfectiousmaterials.Disinfectwith0.05%sodiumhypochlorite.

      *DonotmouthPipettereagents.Ifingested,seekprofessionalassistanceorcontactaPoisonControlCentreimmediately.

      ·Diaminobenzidine(DAB)maybecarcinogenic.Thissolutionmaycauseirritationuponskincontact.Avoidinhalation,ingestion,orskincontactandweargloveswhenhandlingDAB.Ifskincontactoccurs,flushaffectedareawithwater.Spillsmaybe"neutralized"withbleach25,26.Disposeofaccordingtolocalregulations.

      ·Inhalationoringestionofxyleneorthehighlyallergenicfixativeformaldehydeisharmful.Thesematerialsshouldbehandledwithgloves.Prepareinafumehood.Ifskinoreyecontactoccurs,washthoroughlywithcopiousamountsofwater.

      ·Weardisposablegloveswhenhandlinganyhumanmaterialandwhileperformingthestainingprocedure.
      Applications
      ApplicationIHCSelectHRPDetectionSet,(BlockingReagent,BiotinylatedlinkingRiagent&SA_HRP),PredilutedforImmunohistochemistry(Paraffin).
      KeyApplications
      • Immunohistochemistry(Paraffin)
      BiologicalInformation
      SpeciesReactivity
      • Mouse
      • Rabbit
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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