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Millipore/DAB500 | IHC Select® HRP/DAB, 500 Tests/DAB500/1 kit500 tests

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货号: DAB500

品牌: Millipore

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Description
CatalogueNumber	DAB500
Replaces	DAB250
BrandFamily	Chemicon®
TradeName	IHCSelect Chemicon
Description	IHCSelect®HRP/DAB,500Tests
Overview	SummaryandExplanation: Immunohistochemicaltechniqueshavebeeninuseasadjunctivemethodsfortherecognitionofcellsintissuesectionsforatleast50years.Thefirstmethodreporteduseoffluorescentlabelsin19421.In1974,reportsofusingenzymaticlabels,suchashorserADIshperoxidase(HRP)inroutineH&Eparaffinsectionsappeared2,3.Themethodshavesincebecomethe"standardofcare"insurgicalpathologywhenclassicmethodsalonefailtoyieldadefinitivediagnosis4-9. Earlytechniqueswerebasedontheperoxidase-anti-peroxidase(PAP)reaction28,whileimprovementsexploitedthestrongaffinityofavidinforbiotin10.ThistechniqueusesHRP-labeledstreptavidin.Thesecondaryantibodyisconjugatedwithbiotinandthestreptavidin-HRPcomplexreactswiththebiotinonthesecondaryantibodies.Theresultingbiotin-avidin-HRPcomplexcanreactwiththeprimaryantibodyboundtothespecificepitopeofthetargetantigen.TheHRPenzymesofthestreptavidincomplexthencatalyzethesubstrate/chromogenreactiontoformacoloredreactionproduct(browntoblackwhenusingDABasthechromogen)attheantigensite.ABIOLOGicalstainisthenusedtovisualizethewholetissuesection.

ProductInformation
Components	20773/50mL-BlockingReagent:normalgoatseruminphosphatebufferedsaline(PBS)containingcarrierprotein 20775/50mL-SecondaryAntibodies:biotinylatedgoatanti-mouseIgGandgoatanti-rabbitIgGinPBS,containingcarrierprotein 20774/50mL-StreptavidinHRP:DilutedinTrisBufferedSaline 71897/4mL-DABChromogenA:3,3"DiaminobenzidinedilutedinTBS 71898/2x50mL-DABChromogenB:hydrogenperoxidedilutedinTBS,containingTween®20 20785/50mL-Hematoxylin:Mayer"shematoxylincounterstain *20784/2x500mL-20XRinseBuffer:TBS
Detectionmethod	Chromogenic

ProductInformation

Components

*20773/50mL-BlockingReagent:normalgoatseruminphosphatebufferedsaline(PBS)containingcarrierprotein
*20775/50mL-SecondaryAntibodies:biotinylatedgoatanti-mouseIgGandgoatanti-rabbitIgGinPBS,containingcarrierprotein
*20774/50mL-StreptavidinHRP:DilutedinTrisBufferedSaline
*71897/4mL-DABChromogenA:3,3"DiaminobenzidinedilutedinTBS
*71898/2x50mL-DABChromogenB:hydrogenperoxidedilutedinTBS,containingTween®20
*20785/50mL-Hematoxylin:Mayer"shematoxylincounterstain
*20784/2x500mL-20XRinseBuffer:TBS

Detectionmethod

Chromogenic

StorageandShippingInformation
StorageConditions	StABIlityandStorage: Whenstoredat2-8°C,theIHCSelect™ImmunoperoxidaseSecondaryDetectionSystemisstableuptotheexpirationdateprintedonthekitlabel.Donotfreezeorexposetoelevatedtemperatures.Discardanyremainingreagentsaftertheexpirationdate.Donotstorekitcomponentsorperformstaininginstronglight,suchasdirectsunlight. Precautions: ·Pooling,substitutionoralterationofanyreagentmaycauseerroneousresults. Donotmixreagentsfromdifferentlots. Donotallowtheslidestodryatanytimeduringthestainingprocedure. Handleallspecimensandmaterialscomingincontactwiththemaspotentiallyinfectiousmaterials.Disinfectwith0.05%sodiumhypochlorite. DonotmouthPipettereagents.Ifingested,seekprofessionalassistanceorcontactaPoisonControlCenterimmediately. ·Diaminobenzidine(DAB)maybecarcinogenic.Thissolutionmaycauseirritationuponskincontact.Avoidinhalation,ingestion,orskincontactandweargloveswhenhandlingDAB.Ifskincontactoccurs,flushaffectedareawithwater.Spillsmaybe"neutralized"withbleach25,26.Disposeofaccordingtolocalregulations. ·Inhalationoringestionofxyleneorthehighlyallergenicfixativeformaldehydeisharmful.Thesematerialsshouldbehandledwithgloves.Prepareinafumehood.Ifskinoreyecontactoccurs,washthoroughlywithcopiousamountsofwater. ·Weardisposablegloveswhenhandlinganyhumanmaterialandwhileperformingthestainingprocedure.MaterialsRequired: ·PrimaryrabbitormouseIgGantibodies(suchasCHEMICONIHCSelect™PredilutedPrimaryAntibodies) ·Positiveandnegativetissuecontrolslides ·Positivelychargedmicroscopeslides ·Glasscoverslips(24x40mm,No.1) ·Distilledordeionizedwater ·Tween®20 ·Permanentmountingmedium,Eukitt®AmericanHistologyorequivalent ·3%laboratorygradehydrogenperoxide ·10.0%neutralbufferedformalin15 ·Xylene,ACSgrade ·Ethanol,absolute(100%) ·Latex,rubberor100%nitrilegloves(xyleneresistant) ·Timer ·Fumehood ·LightmicroscopeequippedwithPlanAchromatorequivalentlenses ·60°Cdryingoven

StorageandShippingInformation

StorageConditions

StABIlityandStorage:

Whenstoredat2-8°C,theIHCSelect™ImmunoperoxidaseSecondaryDetectionSystemisstableuptotheexpirationdateprintedonthekitlabel.Donotfreezeorexposetoelevatedtemperatures.Discardanyremainingreagentsaftertheexpirationdate.Donotstorekitcomponentsorperformstaininginstronglight,suchasdirectsunlight.

Precautions:

·Pooling,substitutionoralterationofanyreagentmaycauseerroneousresults.

*Donotmixreagentsfromdifferentlots.

*Donotallowtheslidestodryatanytimeduringthestainingprocedure.

*Handleallspecimensandmaterialscomingincontactwiththemaspotentiallyinfectiousmaterials.Disinfectwith0.05%sodiumhypochlorite.

*DonotmouthPipettereagents.Ifingested,seekprofessionalassistanceorcontactaPoisonControlCenterimmediately.

·Diaminobenzidine(DAB)maybecarcinogenic.Thissolutionmaycauseirritationuponskincontact.Avoidinhalation,ingestion,orskincontactandweargloveswhenhandlingDAB.Ifskincontactoccurs,flushaffectedareawithwater.Spillsmaybe"neutralized"withbleach25,26.Disposeofaccordingtolocalregulations.

·Inhalationoringestionofxyleneorthehighlyallergenicfixativeformaldehydeisharmful.Thesematerialsshouldbehandledwithgloves.Prepareinafumehood.Ifskinoreyecontactoccurs,washthoroughlywithcopiousamountsofwater.

·Weardisposablegloveswhenhandlinganyhumanmaterialandwhileperformingthestainingprocedure.MaterialsRequired:
·PrimaryrabbitormouseIgGantibodies(suchasCHEMICONIHCSelect™PredilutedPrimaryAntibodies)
·Positiveandnegativetissuecontrolslides
·Positivelychargedmicroscopeslides
·Glasscoverslips(24x40mm,No.1)
·Distilledordeionizedwater
·Tween®20
·Permanentmountingmedium,Eukitt®AmericanHistologyorequivalent
·3%laboratorygradehydrogenperoxide
·10.0%neutralbufferedformalin15
·Xylene,ACSgrade
·Ethanol,absolute(100%)
·Latex,rubberor100%nitrilegloves(xyleneresistant)
·Timer
·Fumehood
·LightmicroscopeequippedwithPlanAchromatorequivalentlenses
·60°Cdryingoven

Applications
KeyApplications	Immunocytochemistry Immunohistochemistry Immunohistochemistry(Paraffin)
ApplicationNotes	TheIHCSelect™ImmunoperoxidaseSecondaryDetectionSystemisintendedforusewiththeCHEMICONIHCSelect™PredilutedPrimaryAntibodyreagentswhichcontainrabbitandmouseIgGforthequalitativeidentificationofantigensbylightmicroscopyinparaffin-embeddedtissues.TheIHCSelect™ImmunoperoxidaseSecondaryDetectionSystemmayalsobeusedwithotherrabbitormouseIgGprimaryantibodies. GeneralPurposeReagents

BiologicalInformation
SpeciesReactivity	Mouse Rabbit

PhysicochemicalInformation

Dimensions

MaterialsInformation

MaterialsInformation

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Millipore/DAB500 | IHC Select® HRP/DAB, 500 Tests/DAB500/1 kit500 tests

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