The antiserum was produced against synthesized Acetyl-peptide derived from human eIF5A around the Acetylation site of Lys47.
Immunogen Range
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:10000
Specificity
eIF5A (Acetyl-Lys47) Antibody detects endogenous levels of eIF5A protein only when acetylated at Lys47.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using acetylated peptide. The antibody against non-acetylated peptide was removed by chromatography using corresponding non-acetylated peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.