The antiserum was produced against synthesized Acetyl-peptide derived from human ATF5 around the Acetylation site of Lys29.
Immunogen Range
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:10000
Specificity
ATF5 (Acetyl-Lys29) Antibody detects endogenous levels of ATF5 protein only when acetylated at Lys29.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using acetylated peptide. The antibody against non-acetylated peptide was removed by chromatography using corresponding non-acetylated peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
ATF5
Database Links
Gene ID: 22809 Swiss-Prot #: Q9Y2D1
OMIM #: 606398 Unigene #: Hs.9754
Target Modification
Acetyl
Modification Sites
H:K29, M:K29, R:K29
Application Images
Image 1
Western blot analysis of lysate from A549 cells, using ATF5 (Acetyl-Lys29) Antibody.