| Description: | ExoFast™ Exosome Isolation Reagent from other body fluids enables fast and efficient enrichment of intact exosomes from cerebrospinal fluid (CSF), ascitic fluid, amniotic fluid, milk, and saliva with a protocol that is scalable depending on your sample size. By tying up water molecules, the reagent forces less-soluble components such as vesicles out of solution, allowing them to be collected by a short, low-speed centrifugation. The reagent is added to the body fluid, and the solution is incubated at 2–8°C or room temperature (temperature and length of incubation depend on specific body fluid). The precipitated exosomes are recovered by standard centrifugation at 10,000 x g. The pellet is then resuspended in PBS or similar buffer, and the exosomes are ready for downstream analysis or further purification by affinity methods. |
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