| Description: | ABP One Step Cloning Kit is simple, fast, and high efficient reagent which enables directional insertion of any amplified DNA product into any linearized vector at any site. Firstly, the vector is linearized at the cloning site. A small sequence overlapped with each end of the cloning site is added onto the insert through PCR. The insert and the linearized vector, with overlapped sequences of 15 bp – 20 bp on both 5’- and 3’-end, respectively, are mixed in an appropriate ratio and incubated with recombinase Exnase at 50℃ for 5 – 15 min. ABP One Step Cloning Kit is applicable for homologous recombination with 1 – 4 fragments. The kit is independent of DNA ligase, significantly reducing the self-ligated colonies. The highly optimized 5× Clone Master Mix significantly improve the recombination efficiency and the tolerance to impurities, enabling the insert to be used directly for recombination without any treatments to simplify the procedure. |
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