Description: | ROX Reference Dye provides users of the ABI PRISM® 7700 an effective alternative for normalization of fluorescent reporter signal in real-time quantitative PCR or RT-PCR without modifying the instrument’s default analysis parameters. ROX Reference Dye is supplied at 50X concentration. It is composed of a glycine conjugate of 5-carboxy-X-rhodamine, succinimidyl ester (25 μM) in 20 mM Tris- HCl (pH 8.4), 0.1 mM EDTA, 0.01% Tween® 20. It may be used with Platinum® Taq DNA polymerase, Platinum® Quantitative PCR SuperMix-UDG, Platinum® Quantitative RT-PCR ThermoScript™ One-Step System, and SuperScript® One-Step RT-PCR with Platinum® Taq. |