The 2 x Taq PCR Mix with MgCl2 is a premixed solution containing everything needed for successful PCR reaction except specific primers and DNA template. The mix includes high-quality recombinant Taq DNA polymerase, nucleotides and magnesium in a PCR reaction buffer. For the reaction set-up add the PCR Mix (10 or 25 uL) to the primers, template and water for the total reaction volume of 20 or 50 uL.
Quality Control Testing:
Activity and stability tested at 20, 30 and 40 cycles of PCR reactions at 95°C. Free of detectable, non-specific nucleases.Several cycles of freezing/thawing are allowed.
Supplied Product:
Taq DNA Polymerase (0.1 U/uL) in reaction buffer, 4 mM MgCl2, 0.4 mM of each dNTPs (dATP, dCTP, dGTP, dTTP)Water (nuclease free) - 1 mL
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