DNA Marker Middle is useful for determination of DNA fragments cloned in a plasmid DNA. The DNA size marker consists of 14 double-stranded, blunt-end DNA fragments. The size of 2, 686 bp fragment is the same size of pUC18/19. The DNA bands close to each other, 2, 686 and 3,000 bp, 550 bp and 500 bp, are for reference indicators. These DNA fragments were fixed amount in solution of DNA Marker Middle, which makes it possible to estimate amount of DNA.The DNA Marker Middleis supplied in a ready-to-use mixture containing 8 mM EDTA-2Na, 8% glycerol, 0.015% bromphenol blue and 10 mM Tris-HCl (pH 8.0).
Quality Control Testing:
DNA Marker Middle is useful for determination of DNA fragments cloned in a plasmid DNA. The DNA size marker consists of 14 double-stranded, blunt-end DNA fragments.
Recommend Usage:
DNA sample: 5 uL6 X BPB loading dye: 1 uL (addition of 1/5 volume of DNA sample)Mix and load to a well
Supplied Product:
6 x BPB loading dye (EDTA, glycerol and bromphenol blue are contained)
Storage Instruction:
Store at 20-25°C. For long term storage, store at -20°C.
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