i7™ High-Fidelity DNA Polymerase is a genetically engineered, heat stable DNA polymerase which has 5´→3´ polymerase and 3´→5´ exonuclease (proofreading) activities. This enzyme has the high-fidelity, sensitivity and processivity with an error rate ~2.8×102 fold lower than Taq DNA polymerase. i7™ high-fidelity DNA polymerase is ideal for cloning and can be used for long (up to 20 kb) or difficult amplicons. This product is supplied with a proprietary 2.5x i7 PCR reaction buffer containing MgCl2 (final (1x) concentration of 2mM) that also enables efficient amplification of GC rich templates up to 84%.
Product Includes
- i7™ High-Fidelity DNA Polymerase
- 2.5x i7 PCR Buffer with Mg2+
Storage/Handling: Store at -20°C. Product may be shipped on blue ice.