DNase I (Deoxyribonuclease I) is a dialyzed lyophilized powder from bovine pancreas that has been purified by chromatography.
Bovinepancreatic deoxyribonuclease (DNase) is an endonuclease which splitsphosphodiester linkages, preferentially adjacent to a pyrimidinenucleotide yielding polynucleotides with a free hydroxyl group at the 3"position and phosphate group at the 5" position. The optimum pH is 7.8.
DNaseI is activated by bivalent metals, and inhibited by chelating agentssuch as EDTA and SDS. DNase I can be stabilized against proteolyticdigestion by 5mM Ca2+.
Goldbio DNase is tested on bacterial lysates rather thanon pure DNA. Tests on pure DNA are not as rigorous andtend to be biased towardsuccess.
Product SpecificationsDNase IDeoxyribonuclease IDNase I, Bovine Pancreas, >300 Kunitz U/mg
MOLECULAR BIOLOGY GRADE
Unit Definition: One Kunitz Unit will produce an A260 of 0.001 per minute at pH 5.0 at 25°C using DNA, type I or III as the substrate.
Storage/Handling: Store desiccated at -20°C.Allow product to come to room temperature before opening.
Forgreatest stability, it is important that DNase to be dissolved at aconcentration of at least 1 mg/ml in 50% Glycerol with 20mM Tris-HCl, pH7.5, and 1mM MgCl2.
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