SS18 Split CISH Probe is designed for the qualitative detection of human SS18 gene at 18q11.2 in formalin-fixed, paraffin-embedded specimens by chromogenic in situ hybridization (CISH).
Recommend Usage:
The product is ready-to-use. No reconstitution, mixing, or dilution is required. Bring probe to room temperature (18-25°C) and mix briefly before use.
Supplied Product:
Reagent Provided:1. Digoxigenin-labeled polynucleotides targeting sequences mapping in 18q11.2* (chr18:23,109,942-23,262,464) proximal to the SS18 breakpoint region2. Dinitrophenyl-labeled polynucleotides targeting sequences mapping in 18q11.2* (chr18:23,772,255-24,137,169) distal to the SS18 breakpoint region 3. Formamide based hybridization buffer*according to Human Genome Assembly GRCh37/hg19
Storage Instruction:
Store at 2-8°C in an upright position. Return to storage conditions immediately after use.
Note:
The probe is intended to be used in combination with the CISH Implementation Kit 2 (Catalog #: KA5366), which provides necessary reagents for specimen pretreatment and post-hybridization processing.Hybridization signals of digoxigenin-labeled polynucleotides appear dark green distinct dot-shaped (proximal to the SS18 breakpoint region), and dinitrophenyl-labeled polynucleotides appear bright red distinct dot-shaped (distal to the SS18 breakpoint region).Normal situation: In interphases of normal cells or cells without a translocation involving the SS18 gene region, two red/green fusion signals appear.Aberrant situation: One SS18 gene region affected by a translocation is indicated by one separate green signal and one separate red signal. Other signal distribution may be observed in some abnormal samples which might result in a different signal pattern than described above, indicating variant rearrangements. Unexpected signal patterns should be further investigated.
Probe Position:
Regulatory Status:
For research use only (RUO)
Interpretation of Result:
Datasheet:
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Applications
Chromogenic In Situ Hybridization (FFPE Tissue)
Synovial sarcoma tissue section with translocation affecting the 18q11.2 locus as indicated by one non-rearranged red/green fusion signal, one red signal, and one separate green signal indicating the translocation.
Chromogenic In Situ Hybridization (Cells)
Normal interphase cells as indicated by two red/green fusion signals per nucleus.
Application Image
Chromogenic In Situ Hybridization (FFPE Tissue)
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Chromogenic In Situ Hybridization (Cells)
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Gene Information
Entrez GeneID:
6760
Gene Name:
SS18
Gene Alias:
MGC116875,SSXT,SYT,SYT-SSX1,SYT-SSX2
Gene Description:
synovial sarcoma translocation, chromosome 18
Omim ID:
600192
Gene Ontology:
Hyperlink
Other Designations:
SSXT/SSX4v fusion,SYT/SSX4v fusion protein,synovial sarcoma, translocated to X chromosome
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