ROS1 Split CISH Probe is designed for the qualitative detection of human ROS1 gene at 6q22.1 in formalin-fixed, paraffin-embedded specimens by chromogenic in situ hybridization (CISH).
Recommend Usage:
The product is ready-to-use. No reconstitution, mixing, or dilution is required. Bring probe to room temperature (18-25°C) and mix briefly before use.
Supplied Product:
Reagent Provided:1. Digoxigenin-labeled polynucleotides targeting sequences mapping in 6q22.1* (chr6:117,448,964-117,627,255) proximal to the ROS1 breakpoint region2. Dinitrophenyl-labeled polynucleotides targeting sequences mapping in 6q22.1* (chr6:117,659,135-117,871,701) distal to the ROS1 breakpoint region 3. Formamide based hybridization buffer*according to Human Genome Assembly GRCh37/hg19
Storage Instruction:
Store at 2-8°C in an upright position. Return to storage conditions immediately after use.
Note:
The probe is intended to be used in combination with the CISH Implementation Kit 2 (Catalog #: KA5366), which provides necessary reagents for specimen pretreatment and post-hybridization processing.Hybridization signals of digoxigenin-labeled polynucleotides appear dark green colored distinct dotshaped (proximal to the ROS1 breakpoint region), and dinitrophenyl-labeled polynucleotides appear bright red distinct dot-shaped (distal to the ROS1 breakpoint region).Normal situation: In interphases of normal cells or cells without a translocation involving the ROS1 gene region, two red/green fusion signals appear.Aberrant situation: One ROS1 gene region affected by a translocation is indicated by one separate green signal and one separate red signal. Isolated green signals are the result of deletions distal to the ROS1 breakpoint region or are due to unbalanced translocations affecting this chromosomal region. Other signal distribution may be observed in some abnormal samples which might result in a different signal pattern than described above, indicating variant rearrangements. Unexpected signal patterns should be further investigated.
Probe Position:
Regulatory Status:
For research use only (RUO)
Interpretation of Result:
Datasheet:
Download
Applications
Chromogenic In Situ Hybridization (FFPE Tissue)
Lung cancer tissue section with rearrangement of the ROS1 gene as indicated by isolated green signals.
Chromogenic In Situ Hybridization (Cells)
Normal interphase cells as indicated by two red/green fusion signals per nucleus.
Application Image
Chromogenic In Situ Hybridization (FFPE Tissue)
enlarge
Chromogenic In Situ Hybridization (Cells)
enlarge
Gene Information
Entrez GeneID:
6098
Gene Name:
ROS1
Gene Alias:
MCF3,ROS,c-ros-1
Gene Description:
c-ros oncogene 1 , receptor tyrosine kinase
Omim ID:
165020
Gene Ontology:
Hyperlink
Gene Summary:
This proto-oncogene, highly-expressed in a variety of tumor cell lines, belongs to the sevenless subfamily of tyrosine kinase insulin receptor genes. The protein encoded by this gene is a type I integral membrane protein with tyrosine kinase activity. The protein may function as a growth or differentiation factor receptor. [provided by RefSeq
购物车为空，快去购物吧！
4000-520-616
咨询顾问
特别 提示
Download
