MALT1 Split CISH Probe is designed for the qualitative detection of human MALT1 gene at 18q21.32 in formalin-fixed, paraffin-embedded specimens by chromogenic in situ hybridization (CISH).
Recommend Usage:
The product is ready-to-use. No reconstitution, mixing, or dilution is required. Bring probe to room temperature (18-25°C) and mix briefly before use.
Supplied Product:
Reagent Provided:1. Digoxigenin-labeled polynucleotides targeting sequences mapping in 18q21.31-q21.32* (chr18:56,021,766-56,202,885) proximal to the MALT1 breakpoint region2. Dinitrophenyl-labeled polynucleotides targeting sequences mapping in 18q21.32* (chr18:56,557,814-56,724,408) distal to the MALT1 breakpoint region 3. Formamide based hybridization buffer*according to Human Genome Assembly GRCh37/hg19
Storage Instruction:
Store at 2-8°C in an upright position. Return to storage conditions immediately after use.
Note:
The probe is intended to be used in combination with the CISH Implementation Kit 2 (Catalog #: KA5366), which provides necessary reagents for specimen pretreatment and post-hybridization processing.Hybridization signals of digoxigenin-labeled polynucleotides appear dark green distinct dot-shaped (proximal to the MALT1 breakpoint region), and dinitrophenyl-labeled polynucleotides appear bright red distinct dot-shaped (distal to the MALT1 breakpoint region).Normal situation: In interphases of normal cells or cells without a translocation involving the MALT1 gene region, two red/green fusion signals appear. Aberrant situation: One MALT1 gene region affected by a translocation is indicated by one separate green signal and one separate red signal. Other signal distribution may be observed in some abnormal samples which might result in a different signal pattern than described above, indicating variant rearrangements. Unexpected signal patterns should be further investigated.
Probe Position:
Regulatory Status:
For research use only (RUO)
Interpretation of Result:
Datasheet:
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Applications
Chromogenic In Situ Hybridization (Cells)
Normal interphase cells as indicated by two red/green fusion signals per nucleus.
This gene has been found to be recurrently rearranged in chromosomal translocation with two other genes - baculoviral IAP repeat-containing protein 3 (also known as apoptosis inhibitor 2) and immunoglobulin heavy chain locus - in mucosa-associated lymphoid tissue lymphomas. The protein encoded by this gene may play a role in NF-kappaB activation. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq
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