IGH Split CISH Probe is designed for the qualitative detection of human IGH locus at 14q32.33 in formalin-fixed, paraffin-embedded specimens by chromogenic in situ hybridization (CISH).
Recommend Usage:
The product is ready-to-use. No reconstitution, mixing, or dilution is required. Bring probe to room temperature (18-25°C) and mix briefly before use.
Supplied Product:
Reagent Provided:1. Digoxigenin-labeled polynucleotides targeting sequences mapping in 14q32.33* (chr14:106,690,778-106,883,535) distal to the IGH breakpoint region2. Dinitrophenyl-labeled polynucleotides targeting sequences mapping in 14q32.33* (chr14:105,462,169-105,983,969) proximal to the IGH breakpoint region 3. Formamide based hybridization buffer*according to Human Genome Assembly GRCh37/hg19
Storage Instruction:
Store at 2-8°C in an upright position. Return to storage conditions immediately after use.
Note:
The probe is intended to be used in combination with the CISH Implementation Kit 2 (Catalog #: KA5366), which provides necessary reagents for specimen pretreatment and post-hybridization processing.Hybridization signals of digoxigenin-labeled polynucleotides appear dark green distinct dotshaped (distal to the IGH breakpoint region), and dinitrophenyl-labeled polynucleotides appear bright red distinct dot-shaped (proximal to the IGH breakpoint region).Normal situation: In interphases of normal cells or cells without a translocation involving the IGH locus, two red/green fusion signals appear.Aberrant situation: One IGH locus affected by a translocation is indicated by one separate green signal and one separate red signal. Genomic aberrations due to small deletions, duplications or inversions might result in inconspicuous signal patterns. Other signal distribution may be observed in some abnormal samples which might result in a different signal pattern than described above, indicating variant rearrangements.Unexpected signal patterns should be further investigated.
Probe Position:
Regulatory Status:
For research use only (RUO)
Interpretation of Result:
Datasheet:
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Applications
Chromogenic In Situ Hybridization (Cells)
IGH Split CISH Probe hybridized to normal interphase cells as indicated by two red/green fusion signals per nucleus.
Application Image
Chromogenic In Situ Hybridization (Cells)
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Gene Information
Entrez GeneID:
3492
Gene Name:
IGH
Gene Alias:
IGH,IGH.1@,IGHDY1,MGC72071,MGC88774
Gene Description:
immunoglobulin heavy locus
Gene Ontology:
Hyperlink
Gene Summary:
Immunoglobulins recognize foreign antigens and initiate immune responses such as phagocytosis and the complement system. Each immunoglobulin molecule consists of two identical heavy chains and two identical light chains. This region represents the germline organization of the heavy chain locus. The locus includes V (variable), D (diversity), J (joining), and C (constant) segments. During B cell development, a recombination event at the DNA level joins a single D segment with a J segment; this partially rearranged D-J gene is then joined to a V segment. The rearranged V-D-J is then transcribed with the IGHM constant region; this transcript encodes a mu heavy chain. Later in development B cells generate V-D-J-Cmu-Cdelta pre-messenger RNA, which is alternatively spliced to encode either a mu or a delta heavy chain. Mature B cells in the lymph nodes undergo switch recombination, so that the V-D-J gene is brought in proximity to one of the IGHG, IGHA, or IGHE genes and each cell expresses either the gamma, alpha, or epsilon heavy chain. Recombination of many different V segments with several J segments provides a wide range of antigen recognition. Additional diversity is attained by junctional diversity, resulting from the random additional of nucleotides by terminal deoxynucleotidyltransferase, and by somatic hypermutation, which occurs during B cell maturation in the spleen and lymph nodes. Several V, D, J, and C segments are known to be incapable of encoding a protein and are considered pseudogenes. [provided by RefSeq
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