FUS Split CISH Probe is designed for the qualitative detection of translocations involving the human FUS gene at 16p11.2 in formalin-fixed, paraffin-embedded specimens by chromogenic in situ hybridization (CISH).
Recommend Usage:
The product is ready-to-use. No reconstitution, mixing, or dilution is required. Bring probe to room temperature (18-25°C) and mix briefly before use.
Supplied Product:
Reagent Provided:This Probe is composed of:1. Digoxigenin-labeled polynucleotides, which target sequences mapping in 16p11.2* (chr16:30,663,949-30,840,569) distal to the FUS breakpoint region.2. Dinitrophenyl-labeled polynucleotides, which target sequences mapping in 16p11.2* (chr16:31,213,259-31,927,155) proximal to the FUS breakpoint region. 3. Formamide based hybridization buffer.*according to Human Genome Assembly GRCh37/hg19
Storage Instruction:
Store at 2-8°C in an upright position. Return to storage conditions immediately after use.
Note:
The probe is intended to be used in combination with the CISH Implementation Kit 2 (Catalog #: KA5366), which provides necessary reagents for specimen pretreatment and post-hybridization processing.Interpretation of results:Using the CISH Implementation Kit 2 (Cat # KA5366), hybridization signals of Digoxigenin-labeled polynucleotides appear as dark green colored distinct dots (distal to the FUS breakpoint region), and Dinitrophenyl-labeled polynucleotides appear as bright red colored distinct dots (proximal to the FUS breakpoint region).Normal situation: In interphases of normal cells or cells without a translocation involving the FUS gene region, two red/green fusion signals appear.Aberrant situation: One FUS gene region affected by a translocation is indicated by one separate distinct dot-shaped green signal and one separate distinct dot-shaped red signal.Overlapping signals may appear as brown signals. Genomic aberrations due to small deletions, duplications or inversions might result in inconspicuous signal patterns. Other signal patterns than those described above may be observed in some abnormal samples. These unexpected signal patterns should be further investigated.
Probe Position:
Regulatory Status:
For research use only (RUO)
Interpretation of Result:
Datasheet:
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Applications
Chromogenic In Situ Hybridization (FFPE Tissue)
Myxoid liposarcoma tissue section with translocation affecting the 16p11.2 locus as indicated by one non-rearranged red/green fusion signal, one red signal, and one separate green signal indicating the translocation.
Application Image
Chromogenic In Situ Hybridization (FFPE Tissue)
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Gene Information
Entrez GeneID:
2521
Gene Name:
FUS
Gene Alias:
CHOP,FUS-CHOP,FUS1,TLS,TLS/CHOP,hnRNP-P2
Gene Description:
fusion (involved in t(12;16) in malignant liposarcoma)
Omim ID:
137070
Gene Ontology:
Hyperlink
Gene Summary:
This gene encodes a multifunctional protein component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complex. The hnRNP complex is involved in pre-mRNA splicing and the export of fully processed mRNA to the cytoplasm. This protein belongs to the FET family of RNA-binding proteins which have been implicated in cellular processes that include regulation of gene expression, maintenance of genomic integrity and mRNA/microRNA processing. Alternative splicing results in multiple transcript variants. Defects in this gene result in amyotrophic lateral sclerosis type 6. [provided by RefSeq
Other Designations:
FUS-CHOP fusion protein,FUS-CHOP protein fusion,Fusion gene in myxoid liposarcoma,fus-like protein,fus/tls-chop oncogene,heterogeneous nuclear ribonucleoprotein P2,translocated in liposarcoma
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