HPV Screening CISH Probe is designed for the qualitative detection of human papillomavirus (HPV) type 6/11/16/18/31/33/35/39/45/51/52/56/58/59/66/68/82 DNA in formalin-fixed, paraffin-embedded specimens by chromogenic in situ hybridization (CISH).
Recommend Usage:
The product is ready-to-use. No reconstitution, mixing, or dilution is required. Bring probe to room temperature (37°C) and mix briefly before use.
Supplied Product:
Reagent Provided:1.Dinitrophenyl-labeled polynucleotides Digoxigenin-labeled oligonucleotides specific for HPV type 11/16/18/31/33/35/39/45/51/52/56/58/59/66/68/82, which target DNA sequences encoding for the HPV 6/11/16/18/ 31/33/35/39/45/51/52/56/58/59/66/68/82 proteins E6, E7, and/or L1. The probe also targets the respective RNA sequences of E6, E7, and/or L1 proteins, which are expressed during some stages of infection.2. Formamide based hybridization buffer.
Storage Instruction:
Store at 2-8°C in an upright position. Return to storage conditions immediately after use.
Note:
The probe is intended to be used in combination with the CISH Implementation HRP-DAB Kit (Catalog #: KA5367), which provides necessary reagents for specimen pretreatment and post-hybridization processing.The staining pattern in the nucleus can be observed as discrete and dot-shaped signals in case of integrated HPV, or as a strong and homogeneous nuclear staining in case of episomal HPV. A cytoplasmic staining is observed when RNA sequences of HPV are detected. Visualization of signals should be performed using a set of objectives ranging from an at least 200-fold to 630-fold magnification. The presence of the episomal staining pattern is usually detected clearly by an objective with 200-fold magnification, whereas the detection of the integrated HPV pattern requires a greater magnification, preferably 630-fold. Do not evaluate areas of necrosis, overlapping nuclei, over-digested nuclei and nuclei with weak signal intensity.
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