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Abnova/EGFR CISH Probe/1kit/CG0017
  • Abnova/EGFR CISH Probe/1kit/CG0017

Abnova/EGFR CISH Probe/1kit/CG0017

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货号: CG0017
品牌: Abnova
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  • 详情
  • 使用说明
  • 常见问题
      • Specification
      • Gene Info
      • Interactome
      • Pathway
      • Disease
      • Related Products
      • List All
      • Specification
      • Product Description:
      • EGFR CISH Probe is designed for the qualitative detection of human EGFR gene in formalin-fixed, paraffin-embedded specimens by chromogenic in situ hybridization (CISH).
      • Recommend Usage:
      • The product is ready-to-use. No reconstitution, mixing, or dilution is required. Bring probe to room temperature (18-25°C) and mix briefly before use.
      • Supplied Product:
      • Reagent Provided:1. Digoxigenin-labeled polynucleotides targeting sequences mapping in 7p11.2-7p12* (chr7:55,034,991-55,380,617) harboring the EGFR gene2. Formamide based hybridization buffer*according to Human Genome Assembly GRCh37/hg19
      • Storage Instruction:
      • Store at 2-8°C in an upright position. Return to storage conditions immediately after use.
      • Note:
      • The probe is intended to be used in combination with the CISH Implementation Kit 1 (Catalog #: KA5365), which provides necessary reagents for specimen pretreatment and post-hybridization processing.Hybridization signals of digoxigenin-labeled polynucleotides appear as brown- to dark brown colored distinct dots.Normal situation: In interphases of normal cells or cells without an amplification involving the 7p11.2 band, two distinct dot-shaped brown signals per nuclei appear.Aberrant situation: In a cell with amplification of the EGFR gene locus or aneuploidy of chromosome 7, multiple copies of the brown signal or brown signal clusters will be observed. Other signal distribution may be observed in some abnormal samples which might result in a different signal pattern than described above, indicating variant rearrangements. Unexpected signal patterns should be further investigated.
      • Probe Position:
      • Regulatory Status:
      • For research use only (RUO)
      • Interpretation of Result:
      • Datasheet:
      • PDF DownloadDownload
      • Applications
      • Chromogenic In Situ Hybridization (FFPE Tissue)
      • Chromogenic <i>In Situ</i> Hybridization (FFPE Tissue)
      • Normal nuclei each with two EGFR signals.
      • Chromogenic In Situ Hybridization (Cells)
      • Chromogenic <i>In Situ</i> Hybridization (Cells)
      • NSCLC cancer cells with multiple EGFR signals in sputum sample.
      • Application Image
      • Chromogenic In Situ Hybridization (FFPE Tissue)
      • Chromogenic <i>In Situ</i> Hybridization (FFPE Tissue)
      • enlarge
      • Chromogenic In Situ Hybridization (Cells)
      • Chromogenic <i>In Situ</i> Hybridization (Cells)
      • enlarge
      • Gene Information
      • Entrez GeneID:
      • 1956
      • Gene Name:
      • EGFR
      • Gene Alias:
      • ERBB,ERBB1,HER1,PIG61,mENA
      • Gene Description:
      • epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
      • Omim ID:
      • 131550, 211980
      • Gene Ontology:
      • Hyperlink
      • Gene Summary:
      • The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq
      • Other Designations:
      • avian erythroblastic leukemia viral (v-erb-b) oncogene homolog,cell growth inhibiting protein 40,cell proliferation-inducing protein 61,epidermal growth factor receptor
      • Interactome
      • Interactome
      • Gene Pathway
      • Adherens junction
      • Bladder cancer
      • Calcium signaling pathway
      • Colorectal cancer
      • Cytokine-cytokine receptor interaction
      • Dorso-ventral axis formation
      • Endocytosis
      • Endometrial cancer
      • Epithelial cell signaling in Helicobacter pylori infection
      • ErbB signaling pathway
      • Focal adhesion
      • Gap junction
      • Glioma
      • GnRH signaling pathway
      • MAPK signaling pathway
      • Melanoma
      • Non-small cell lung cancer
      • Pancreatic cancer
      • Pathways in cancer
      • Prostate cancer
      • Regulation of actin cytoskeleton
      • Related Disease
      • Adenocarcinoma
      • Adenocarcinoma, Bronchiolo-Alveolar
      • Adenocarcinoma, Mucinous
      • Adenocarcinoma, Papillary
      • Anus Neoplasms
      • Asthma
      • Asthma
      • Astrocytoma
      • Atherosclerosis
      • Barrett Esophagus
      • Bile Duct Neoplasms
      • Biliary Tract Neoplasms
      • Bipolar Disorder
      • Brain Neoplasms
      • Breast cancer
      • Breast Neoplasms
      • Bronchial Hyperreactivity
      • Carcinoma
      • Carcinoma, Adenosquamous
          • ... see more
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