CDKN2A/CEN9 CISH Probe is designed for the qualitative detection of human CDKN2A gene deletions and the detection of chromosome 9 classical satellites III in formalin-fixed, paraffin-embedded specimens by chromogenic in situ hybridization (CISH).
Recommend Usage:
The product is ready-to-use. No reconstitution, mixing, or dilution is required. Bring probe to room temperature (18-25°C) and mix briefly before use.
Supplied Product:
Reagent Provided:This Probe is composed of:1. Digoxigenin-labeled polynucleotides, which target sequences mapping in 9p21.3* (chr9:21,742,629-22,056,853) harboring the CDKN2A gene region.2. Dinitrophenyl-labeled polynucleotides, which target sequences mapping in chromosomal region 9q12 specific for the classical satellite III region D9Z3 of chromosome 9. 3. Formamide based hybridization buffer.*according to Human Genome Assembly GRCh37/hg19
Storage Instruction:
Store at 2-8°C in an upright position. Return to storage conditions immediately after use.
Note:
The probe is intended to be used in combination with the CISH Implementation Kit 2 (Catalog #: KA5366), which provides necessary reagents for specimen pretreatment and post-hybridization processing.Interpretation of results:Using the CISH Implementation Kit 2 (Cat # KA5366), hybridization signals of Digoxigenin-labeled polynucleotides appear as dark green colored distinct dots (CDK4 gene region), and Dinitrophenyl-labeled polynucleotides appear as bright red colored distinct dots (CEN 12).Normal situation: In interphases of normal cells or cells without an amplification involving the CDK4 gene region, two distinct dot-shaped green and two distinct dot-shaped red signals appear.Aberrant situation: In cells with an amplification of the CDK4 gene region, an increased number of green signals or green signal clusters will be observed.Overlapping signals may appear as brown signals. Other signal patterns than those described above may be observed in some abnormal samples. These unexpected signal patterns should be further investigated.
Probe Position:
Regulatory Status:
For research use only (RUO)
Interpretation of Result:
Datasheet:
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Applications
Chromogenic In Situ Hybridization (Cells)
Normal interphase cells as indicated by two red and two green signals per nucleus.
This gene generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, MDM1, a protein responsible for the degradation of p53. In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by this gene, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 control. This gene is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene. [provided by RefSeq
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