Purity | Partiallypurifiedrecombinantcaspase3withafulllengthHIS-6-tag.Approximately10-20%caspase2protein.TheremainderisE.Coliproteins.Approximately0.8-2mgtotalprotein/Uofactivity;E.Coliproteinshavenoreactivity. |
SpecificActivity | 25U:Whereoneunitoftherecombinantcaspase-2istheenzymeactivitythatcleaves1nmolofthecaspasesubstrateVDVAD-pNA(pNA:pnitroanaline)perhourat37oCinareactionsolutioncontaining50mMHepes,pH7.2,50mMNaCl,0.1%Chaps,10mMEDTA,5%Glycerol,and10mMDTT. |
EntrezGeneNumber | |
EntrezGeneSummary | Thisgeneencodesaproteinwhichisamemberofthecysteine-asparticacidprotease(caspase)family.Sequentialactivationofcaspasesplaysacentralroleintheexecution-phaseofcellapoptosis.Caspasesexistasinactiveproenzymeswhichundergoproteolyticprocessingatconservedasparticresiduestoproducetwosubunits,largeandsmall,thatdimerizetoformtheactiveenzyme.Theproteolyticcleavageofthisproteinisinducedbyavarietyofapoptoticstimuli.Alternativesplicingofthisgeneresultsinmultipletranscriptvariantsthatencodedifferentisoforms. |
GeneSymbol | - CASP2
- ICH1
- NEDD2
- NEDD-2
- ICH-1L/1S
- CASP-2
- ICH-1L
- EC3.4.22.55[Contains:Caspase-2subunitp18
- Caspase-2subunitp13
- Caspase-2subunitp12].
|
UniProtNumber | |
UniProtSummary | FUNCTION:SwissProt:P42575#InvolvedintheactivationcascadeofcaspasesresponsIBLeforapoptosisexecution.Mightfunctionbyeitheractivatingsomeproteinsrequiredforcelldeathorinactivatingproteinsnecessaryforcellsurvival. SIZE:452aminoacids;50685Da SUBUNIT:Heterotetramerthatconsistsoftwoanti-parallelarrangedheterodimers,eachoneformedbyap18subunitandap12subunit.InteractswithLRDD. TISSUESPECIFICITY:Expressedinlargeramountsintheembryoniclung,liverandkidneythanintheheartandbrain.Intheadultshigherlevelexpressionisseenintheplacenta,lung,kidney,pancreasthanintheheart,brain,liverandskeletalmuscle. PTM:Thematureproteasecanprocessitsownpropeptide,butnotthatofothercaspases. SIMILARITY:SwissProt:P42575##BelongstothepeptidaseC14family.&Contains1CARDdomain. |