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Millipore/CC120 | Caspase 9, recombinant human active/CC120/25 units
  • Millipore/CC120 | Caspase 9, recombinant human active/CC120/25 units

Millipore/CC120 | Caspase 9, recombinant human active/CC120/25 units

价格: ¥3072.00 市场价: 5120.00

货号: CC120
品牌: Millipore
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    • Description
      CatalogueNumberCC120
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionCaspase9,recombinanthumanactive
      OverviewCaspase-9isamemberoftheinterleukin-1betaconvertingenzyme(ICE)familyofcysteineproteases.Similartoothercaspases,caspase-9alsoexistsincellsasaninactiveproenzyme.Duringtheinitiationofapoptosistheproenzymeisprocessedataspartateresiduestoformactivecaspase-9.Asoneoftheinitiatorcaspases,activecaspase-9functionstotriggertheactivationofdownstreameffectorcaspasesandfurtherleadtodisassemblyofcellstructures.

      Thepartiallypurifiedactiverecombinanthumancaspase-9withfull-lengthHIS-6tagwasexpressedinE.coli.Theexpressedcaspase-9spontaneouslyundergoesautoprocessingtoyieldthesubunitscharacteristicofthenativeenzyme.Incombinationwithcaspase-9activityassays,theactiverecombinantcaspase-9isusefulinBIOLOGicalscreeningofcaspaseinhibitors.Therecombinantenzymecanalsobeusedasapositivecontrolincaspase-9assays.Caspase9isknowntohavestrongreactivitywithothersequencepeptides,especiallyDEVD-pNA.
      AlternateNames
      • APAF-3
      • ICE-LAP6
      • Mch-6
      ProductInformation
      PresentationLyophilized.Reconstitutewith25μlofPBS+15%glycerol.
      StorageandShippingInformation
      StorageConditionsMaintainat-70°Cinundilutedaliquotsfor3-6monthsfromdateofreceipt.Avoidrepeatedfreeze/thawcycles.
      Applications
      KeyApplications
      • ActivityAssay
      ApplicationNotesEnzymeispreparedasanactivitystandardonly,anyotherapplicationnottested.Caspase-9issubjecttoauto-activationthusactivecaspasesubunitsandpartiallyactiveintermediateswillbeseen.Bandsofapproximately45kDa(pro),35kDa(partiallyactive)and18kDa+12kDa(largeandsmallsubunits)maybeseenbywesternblotdependingupontheantibodyused.Thesmallsubunitisextremelydifficulttodetectinmostwesterns.

      Activation:incubate1-5μLofstockinPBSpH7.5(100mMHEPEScanbeusedaswell)plus10mMATPand5mMDTT(addedjustbeforeuse)for30minutesat37°C.
      BiologicalInformation
      Purity10-20%caspase-9proteinbygelanalysis.AlsocontainsE.coliproteins.Approximately,0.8-2μgtotalprotein/unitofactivity.
      SourceHuman
      SpecificActivityOneunitoftherecombinantcaspase-9istheenzymeactivitythatcleaves1nmolofthecaspasesubstrateLEHD-pNA(pNA:pnitroanaline)perhourat37°Catsaturatedsubstrateconcentrations.
      EntrezGeneNumber
      EntrezGeneSummaryThisgeneencodesamemberofthecysteine-asparticacidprotease(caspase)family.Sequentialactivationofcaspasesplaysacentralroleintheexecution-phaseofcellapoptosis.Caspasesexistasinactiveproenzymeswhichundergoproteolyticprocessingatconservedasparticresiduestoproducetwosubunits,largeandsmall,thatdimerizetoformtheactiveenzyme.ThisproteinisprocessedbycaspaseAPAF1;thisstepisthoughttobeoneoftheearliestinthecaspaseactivationcascade.Alternativesplicingresultsintwotranscriptvariantswhichencodedifferentisoforms.
      GeneSymbol
      • CASP9
      • CASP-9
      • ICE-LAP6
      • MCH6
      • APAF3
      • CASPASE-9c
      • OTTHUMP00000044594
      • APAF-3
      • EC3.4.22.62[Contains:Caspase-9subunitp35
      • Caspase-9subunitp10].
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P55211#Isoform2lacksactivityisandominant-negativeinhibitorofcaspase-9.
      SIZE:416aminoacids;46281Da
      SUBUNIT:Heterotetramerthatconsistsoftwoanti-parallelarrangedheterodimers,eachoneformedbya35kDa(p35)anda10kDa(p10)subunit.Caspase-9andAPAF1bindtoeachotherviatheirrespectiveNH2-terminalCED-3homologousdomainsinthepresenceofcytochromeCandATP.InteractswiththeinhibitorsBIRC2,BIRC4,BIRC5andBIRC7.
      TISSUESPECIFICITY:Ubiquitous,withhighestexpressionintheheart,moderateexpressioninliver,skeletalmuscle,andpancreas.Lowlevelsinallothertissues.
      PTM:CleavagesatAsp-315bygranzymeBandatAsp-330bycaspase-3generatethetwoactivesubunits.Caspase-8and-10canalsobeinvolvedintheseprocessingevents.
      SIMILARITY:SwissProt:P55211##BelongstothepeptidaseC14family.&Contains1CARDdomain.
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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