GoldBio/DL39 (DE3) Chemically Competent E. coli Cells/5 x 200 μL/CC-104-5x200

GoldBio’s DL39 (DE3) Chemically Competent E. colicells are engineered with a T7 expression system to transform and express proteins in order to label residues such as phenylalanine or leucine. DL39 (DE3) Chemically Competent E. colicells are deficient in the aromatic (TyrB), branched-chain (JIvE), and aspartate (AspC) transaminases and are auxotrophic for aspartic acid, isoleucine, leucine, phenylananine, tyrosine, and valine residues.

Product SpecificationsCompetent cell type: Chemically CompetentDerivative of: DL39Species: E. coliTransformation efficiency: ≥1 x 10⁷ cfu/µg pUC19 DNABlue/white screening: Yes

Storage/Handling: This product may be shipped on dry ice. DL39 (DE3) Chemically Competent E. coli cells should be stored at -80°C, pUC19 Control DNA should be stored at -20°C and recovery medium should be stored at 4°C immediately upon arrival. When stored under the recommended conditions and handled correctly, these products should be stable for at least 1 year from the date of receipt.

Genomic Features

• >1 x 10⁷ cfu/µg efficiency
• Deficient in the aromatic (TyrB), branched-chain (JIvE), and aspartate (AspC) transaminases.
• Contains the T7 expression system

GenotypeF-, λ-, aspC13, fnr-25, rph-1, ilvE12, tyrB507, λDE3

Reagents Needed for One Reaction

• DL39 (DE3) chemically competent cells: 50 µl
• DNA (or pUC19 Control, 10 pg/µl): 1 µl
• Recovery medium: 1 ml

Quality ControlTransformation efficiency is tested by using the pUC19 control DNA supplied with the kit and using the protocol given below. Transformation efficiency should be ≥1 x 10⁷ CFU/µg pUC19 DNA. Untransformed cells are tested for appropriate antibiotic sensitivity.

General Guidelines

• Handle competent cells gently as they are highly sensitive to changes in temperature or mechanical lysis caused by pipetting.
• Thaw competent cells on ice and transform cells immediately following thawing. After adding DNA, mix by tapping the tube gently. Do not mix cells by pipetting or vortexing.

Calculation of Transformation EfficiencyTransformation Efficiency (TE) is defined as the number of colony forming units (cfu) produced by transforming 1 µg of plasmid into a given volume of competent cells.

• TE = Colonies/µg/Dilution
  • Colonies = the number of colonies counted
  • µg = amount of DNA transformed in µg
  • Dilution = total dilution of the DNA before plating

Example: Transform 1 µl of (10 pg/µl) control plasmid into 25 µl of cells, add 975 µl of Recovery Medium. Dilute 10 µl of this in 990 µl of Recovery Medium and plate 50 µl. Count the colonies on the plate the next day. If you count 250 colonies, the TE is calculated as follows:Colonies = 250µg of DNA = 0.00001 Dilution = 10/1000 x 50/1000 = 0.0005 TE = 250/0.00001/0.0005 = 5.0 × 10¹⁰