Chemically competent E. coli cells suitable for high efficiency transformation and isolation of plasmid clones containing repeat elements.
Highlights
Recommended host strain for cloning genes into retroviral/ lentiviral vectors
Recommended for cloning of direct repeats and inverted repeat sequences
Reduced recombination of cloned DNA (recA1)
Efficient transformation of methylated DNA derived from eukaryotic sources or unmethylated DNA derived from PCR, cDNA and many other sources [mcrAΔ(mrr-hsdRMS-mcrBC)]
Activity of nonspecific endonuclease I (endA1) eliminated for highest quality plasmid preparations
Resistance to phage T1 (fhuA)
Suitable for blue/white screening with vectors capable of alpha-complementation