Chemically competent E. coli B cells engineered to form proteins containingdisulfide bonds in the cytoplasm. Suitable for T7 promoter driven protein expression of toxic proteins.
Highlights
Engineered E. coli B strain to promote disulfide bond formation in the cytoplasm
Constitutivelyexpresses a chromosomal copy of the disufide bond isomerase DsbC
DsbC promotes the correction of mis-oxidized proteins into their correct form (1,3)
The cytoplasmic DsbC is also a chaperone that can assist in the folding of proteins that do not require disulfide bonds (4)
Expresses a chromosomal copy of T7 RNAP
Inactive mutant lysozyme expressed from miniF plasmid
Allows for the expression of toxic proteins
Enhanced BL21 derivative
Resistance to phage T1 (fhuA2), Cam, Nit, Spec, and Str**Resistance to low levels of streptomycin may be observed.