Chemically competent E. coli B cells engineered to form proteins containing disulfide bonds in the cytoplasm. Suitable for T7 promoter driven protein expression.
Highlights
Engineered E. coli B strain to promote disulfide bond formation in the cytoplasm
Constitutively expressesa chromosomal copy of the disufide bond isomerase DsbC
DsbC promotes the correction of mis-oxidized proteins into their correct form (1,3)
The cytoplasmic DsbC is also a chaperone that can assist in the folding of proteins that do not require disulfide bonds (4)
Enhanced BL21 derivative
Expresses a chromosomal copy of T7 RNAP
Activity of nonspecific endonuclease I (endA1) eliminated for highest quality plasmid preparations
Deficient in proteases Lon and OmpT
Resistance to phage T1 (fhuA2), Nit, Spec, Str**Resistance to low levels of streptomycin may be observed.