Chemically competent E. coli K12 cells engineered to form proteins containing disulfide bonds in the cytoplasm. Suitable for T7 promoter driven protein expression.
Highlights
Engineered E. coli K12 to promote disulfide bond formation in the cytoplasm
Constitutivelyexpresses a chromosomal copy of the disufide bond isomerase DsbC
DsbC promotes the correction of mis-oxidized proteins into their correct form (1,3)
The cytoplasmic DsbC is also a chaperone that can assist in the folding of proteins that do not require disulfide bonds (4)
Expresses a chromosomal copy of T7 RNAP
Tight control of expression by lacIq allows potentially toxic genes to be cloned