NEB 10-beta electrocompetent E. coli cells are optimized for high efficiency transformation by electroporation. These cells are ideal for DNA library constructions and all cloning purposes.
Highlights
DH10B™ derivative
Accomodation of large plasmids including BAC and cosmid constructs
Efficient transformation of methylated DNA derived from eukaryotic sources and unmethylated DNA derived from PCR, cDNA and other sources
Activity of nonspecific endonuclease I eliminated for highest quality plasmid preparations (endA1)
Suitable for blue/white screening by α-complementation of the β-galactosidase gene (Φ80ΔlacZM15)