Chemically competent E. coli cells suitable for high efficiency transformation in a wide variety of applications.
Highlights
DH10B™ derivative
Efficient transformation of methylated DNA derived from eukaryotic sources or unmethylated DNA derived from PCR, cDNA and many other sources [mcrAΔ(mrr-hsdRMS-mcrBC)]
Activity of nonspecific endonuclease I (endA1) eliminated for highest quality plasmid preparations
Resistance to phage T1 (fhuA)
Suitable for blue/white screening without IPTG by a-complementation of the b-galactosidase gene
Reduced recombination of cloned DNA (recA1)
Clone large plasmids and BACs
Free of animal products
K12 Strain
Transformation Efficiency
For C3019I and C3019H: 1–3 x 109cfu/µg pUC19 DNAFor C3019P: 1–3 x 108cfu/µg pUC19 DNA