Chemically competent E. coli cells suitable for subcloning efficiencytransformations, such as plasmid transformation or routine subcloning(e.g,. inserting a 1 kb fragment into a 2 kb vector). The efficiency ofthese cells is not sufficiently high for most other cloningapplications. For that, we recommend NEB® 5-alpha Competent E. coli(High Efficiency) (NEB #C2987) which has 1,000-fold highertransformation efficiency.
Highlights
Activity of nonspecific endonuclease I (endA1) eliminated for highest quality plasmid preparations
Efficient transformation of unmethylated DNA derived from PCR, cDNA and many other sources (hsdR)
Reduced recombination of cloned DNA (recA1)
Resistance to phage T1 (fhuA2)
Suitable for blue/white screening by α-complementation of the β-galactosidase gene