Chemically competent E. coli cells suitable for high efficiency transformation in a wide variety of applications. Available in 5 convenient formats:
- Single-use 50 μl vials
- 200 μl vials
- 96-well plate
- 384-well plate
- 12 x 8-tube strips: packed in 12 x 8-Strip Microplate Cluster Tube (Costar™ Cluster Tube System)
For C2987H, C2987I, C2987P and C2987U, SOC Outgrowth Medium is provided. For C2987R, Outgrowth Medium 1.5 is provided.
Highlights
- DH5α™ derivative
- Free of animal products
- Efficient transformation of unmethylated DNA derived from PCR, cDNA and many other sources (hsdR)
- Activity of nonspecific endonuclease I (endA1) eliminated for highest quality plasmid preparations
- Resistance to phage T1 (fhuA2)
- Suitable for blue/white screening by α-complementation of the β-galactosidase gene
- Reduced recombination of cloned DNA (recA1)
- K12 Strain
Transformation Efficiency
For C2987H, C2987I, C2987P and C2987U: 1- 3 x 109cfu/μg pUC19 DNAFor C2987R: 1- 5 x 108cfu/μg pUC19 DNA Genotype
fhuA2Δ(argF-lacZ)U169 phoA glnV44 Φ80Δ(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17 - This product is related to the following categories:
- Cloning Competent Cell Strains Products
- This product can be used in the following applications:
- USER® Cloning,
- Applications of USER® and Thermolabile USER II Enzymes,
- High-throughput cloning and automation solutions,
Transformation