Methyltransferase deficient chemically competentE. colicells suitable for growth of plasmids free of Dam and Dcm methylation. Note that dam- strains are not recommended as a host for primary cloning/ligation. The dam mutation can result in an increased mutation rate in the cell and a reduction in the transformation efficiency. DNA should be maintained in a dam+ strain unless there is a specific need for DNA free of Dam or Dcm methylation.
Highlights
Grow plasmids free of Dam and Dcm methylation
Free of animal products
Phage T1 resistant (fhuA31)
Activity of nonspecific endonuclease I (endA1) eliminated for highest quality plasmid preparations