CFDA, SE is a fixable-cell-permeant, fluorescein-based tracer for very long-term cell labeling. CFDA, SE passively diffuse into cells and it is nonfluorescent until the acetate groups are cleaved by intracellular esterases to yield the highly fluorescent, amine-reactive fluorophore. Upon reaction with amine-containing residues of intracellular proteins, this probe is well retained in cells.
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