Gel Loading Dye, Purple (6X) is apre-mixed loading buffer which contains a combinationof two dyes, Dye 1 (pink/red) and Dye 2(blue). The red dye serves as the tracking dye forboth agarose and non-denaturing polyacrylamidegel electrophoresis. The two dyes separate upongel electrophoresis; the red band is the majorindicator and migrates similarly to BromophenolBlue on agarose gels. Specifically chosen, thisdye does not leave a shadow under UV light. Thissolution contains SDS, which often results insharper bands, as some restriction enzymes areknown to remain bound to DNA following cleavage.EDTA is also included to chelate magnesium(up to 10 mM) in enzymatic reactions, therebystopping the reaction. The dye also contains Ficoll,which creates brighter and tighter bands whencompared to glycerol loading dyes. This product is packaged as 4x1 ml vials.Attention SYBR® Safe and GelRed™ dye users: Due to an increased concentration of SDS* in B7024S, NEB recommends using Gel Loading Dye, Purple, No SDS (6X) (NEB# B7025S) instead.*Compared to NEB# B7021 and NEB# B7022.Comparison of dye frontsNucleic acid samples were run on an agarosegel, with either Purple or Blue loading dyes.Both dyes ran at the same speed.UV shadow comparisonThe new Gel Loading Dye, Purple (6X) (Lane 1) included in the Quick-Load Purple 1kb DNA Ladder does not cast a UV shadow over the underlying bands, unlike the Gel Loading Dye, Blue (6X) (Lane 2).
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