Bicine is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, bis(2-hydroxyethyl)amine buffer that is useful for a pH range of 7.6 – 9.0. It is commonly used in protein crystallization, in the study of enzymatic reactions, and for electrophoresis. It is used in thin layer ion exchange chromatography for protein resolution, as well as a multiphasic buffer system for SDS-PAGE of proteins. It is soluble in water at 0°C up to 1.1 M. Bicine forms a complex with most common metals so stability constants and concentrations should be taken into consideration when choosing this buffer.
Product SpecificationsBicine;N,N-bis(2-hydroxyethyl)glycine;di(hydroxyethyl)glycine;N,N- bis(hydroxyethyl)aminoaceticacid
ULTRA PURE GRADE
Formula: C6H13NO4
MW: 163.17 g/mol
PubChem Chemical ID: 8761
Storage/Handling: Store at roomtemperature.
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