Granzyme B is a serine protease mostly secreted by cytotoxic T lymphocyte (CTL) and natural killer (NK) cells. It is one of the major mediators of caspase-dependent apoptosis. Recent studies showed that Granzyme B can also be secreted by various non-cytotoxic cells and involved in degradation of extracellular matrix proteins inducing inflammation and impairing wound healing. Thus if intracellular activity of Granzyme B is mainly related to apoptosis its extracellular activity has been linked to arthritis, vascular pathologies, and other diseases. The SensoLyte® 520 Granzyme B Assay Kit provides a convenient assay for screening of enzyme inhibitors or for continuous assay of Granzyme B activity using a novel internally quenched 5-FAM/QXLTM FRET substrate. Upon cleavage by Granzyme B, the FRET substrate will be separated into two fragments resulting in release of 5-FAM fluorescence which can be monitored at excitation /emission= 490 nm/520 nm. The kit can be used to detect the activity of Granzyme B enzyme in biological samples and purified enzyme preparations. This assay can detect as low as 0.1 ng/mL active granzyme B.The kit contains: • QXL® 520/ 5-FAM-based FRET peptide substrate (Ex/Em=490/520 nm upon cleavage) • Assay buffer• Recombinant Human Granzyme B• Inhibitor • Fluorescence reference standard for calibration • A detailed protocol Kit size: 100 assays (96-well plate)
Order #:
AS-72261
Unit Size:
1 kit
Supplier:
AnaSpec
shipping:
Blue ice
storage:
-20°C
restrictions:
Only available in Germany and selected European countries.
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