Granzyme A is the most abundant serine protease secreted by cytotoxic T lymphocyte (CTL) and natural killer (NK) cells. Granzyme A enters pathogen-containing and tumor cells by perforin pathway and induces caspase-independent apoptosis. Thus cells that are resistant to the caspases or Granzyme B might be sensitive to Granzyme A.Recent studies showed that Granzyme A activates a novel programmed cell death pathway that begins in the mitochondrion. Granzyme A also targets several important nuclear proteins for degradation including histones and DNA damage repair proteins. Granzyme A has proinflammatory activity inducing certain cytokines secretion. SensoLyte® 520 Granzyme A Assay Kit provides a convenient assay for screening of enzyme inhibitors or for detecting the activity of enzyme using a novel internally quenched 5-FAM/QXLTM FRET substrate. Upon cleavage by granzyme A, the FRET substrate will be separated into two fragments resulting in release of 5-FAM fluorescence which can be monitored at excitation /emission= 490 nm/520 nm. The long wavelength fluorescence of 5-FAM is less interfered by the autofluorescence of components in biological samples and test compounds. This kit can be used to detect the activity of Granzyme A enzyme in biological samples and purified enzyme preparations.The kit contains: • QXL® 520/ 5-FAM-based FRET peptide substrate (Ex/Em=490/520 nm upon cleavage) • Assay buffer• Inhibitor • Fluorescence reference standard for calibration • A detailed protocol Kit size: 100 assays (96-well plate)
Order #:
AS-72260
Unit Size:
1 kit
Supplier:
AnaSpec
shipping:
Blue ice
storage:
-20°C
restrictions:
Only available in Germany and selected European countries.
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