| Description: | BPE (B-Phycoerythrin), a fluorescent protein, belongs to the phycobiliproteins family, a family of highly soluble and fluorescent proteins derived from cyanobacteria and eukaryotic algae. BPE is made ofα, β and γ subunits and is present as ( αβ)6γ. It has absorption bands with peaks at 545 nm (εM 2.41 X 106 M-1cm-1), and 563 (εM 2.33 X 106 M-1cm-1) and has maximal emission at 578 nm. BPE and the closely related RPE are the most intensely fluorescentphycobiliproteins and it is 10-20 times brighter than conventional organic fluorophores. Additionally, phycobiliproteins are more photostable than their organic counterparts. BPE labeled streptavidin is widely used in flow cytometry, live cell staining, and multi-color immunofluorescent staining. |
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