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Millipore/AG400 | BLACK-GOLD® II Stain/AG400/150 mg

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货号: AG400

品牌: Millipore

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Description
CatalogueNumber	AG400
Replaces	AG390
TradeName	BLACK-GOLD
Description	BLACK-GOLD®IIStain
Overview	Black-Gold®IIstainisanaurohalophosphatecomplexwhichstainsspecificallyformyelinwithinthecentralnervoussystem.Black-GoldIIstainingprovideshighresolution,highcontrast,ashorthistochemicalprocessingtime,andhighreproducibility.Black-GoldIIstainisanewandimprovedversionofitspredecessor,Black-Goldstain(discontinued).TheadvantagesofMillipore’sBlack-GoldIIoverBlack-GoldIstainarethatitismorereADIlysoluble,canbeutilizedatahigherconcentration,producesamoreuniformandconsistentstaining,takeslesstimetostain,anddoesnotrequirepost-stainingintensificationsteps. TheuseofBlack-GoldIIstainistailoredtostudiesusingformalin-fixed,non-solventprocessedtissue.Thetechniquestainslargemyelinatedtractsdarkred-brown,whiletheindividualmyelinatedfibersappearblack.Thisnoveltracercanbeusedtolocalizebothnormalandpathologicalmyelin.Black-GoldIIstaincandemonstrateandcharacterizespecificmyelinchangesassociatedwithexposuretodiverseneurotoxicantsincludingkainicacid,methamphetamine,acrylamide,domoicacid,3-nitropropionicacid,Fluoro-Gold™tracerandisoniazid. BlackGoldIIPurity:Nodetectableamountofuncomplexedgoldwasfound. BlackGoldIIIllumination:Brightfieldordarkfield. BlackGoldIISolubility:Freelysolubleinwater,saline,ordiluteacids.

ProductInformation
Components	Black-GoldII:150mgdrypowder,yellowincolor.
Presentation	SeeComponents

StorageandShippingInformation
StorageConditions	Thekitcanbestoredatroomtemperature.Ideally,theBlack-GoldIIpowdershouldbekeptinadesiccator,becauseofitshygroscopicnature.Thedrypowder,ifunopenedandstoredproperly,isusableforuptooneyearfromdateofreceipt.OnceresUSPendedtheBlack-GoldIIstainingsolutionshouldbestoredinthedarkat4°Candisgoodforupto3months. TOXICITY:Althoughthecompoundappearstobeoflowtoxicity,ithasnotbeenextensivelyevaluatedandthereforeroutinelaboratorycautionshouldbeexercised.Notintendedforhumanconsumption.

StorageandShippingInformation

StorageConditions

Thekitcanbestoredatroomtemperature.Ideally,theBlack-GoldIIpowdershouldbekeptinadesiccator,becauseofitshygroscopicnature.Thedrypowder,ifunopenedandstoredproperly,isusableforuptooneyearfromdateofreceipt.OnceresUSPendedtheBlack-GoldIIstainingsolutionshouldbestoredinthedarkat4°Candisgoodforupto3months.

TOXICITY:Althoughthecompoundappearstobeoflowtoxicity,ithasnotbeenextensivelyevaluatedandthereforeroutinelaboratorycautionshouldbeexercised.Notintendedforhumanconsumption.

Applications
KeyApplications	Immunohistochemistry
ApplicationNotes	TissueFixation: Tissuecanbefixedwitheitherformalinor4%PFA.Typicallyanimalsareperfusedwithneutralphosphatebuffered10%formalin(or4%formaldehyde)viatheascendingaorta.Thebrainsarethenremovedandfixedovernightinthesamefixativesolution.Longerfixationtimesaregenerallynotrecommended.Forcryosections,thebrainsmaybeprotectedthroughtreatmentwithsucrosesolution(20-30percent).Subsequentlyfrozenbraintissueiscutonafreezingslidingmicrotome.Note:Black-GoldIIwillnotstainparaffinembeddedorunfixedtissue. Sectioning: Eitherfrozenorfixed,non-frozenvibratomesectionscanbeusedandshouldbecutatathicknessof20-40μm.Thesectionsarethentypicallymountedonpositivelychargedslides.Thesectionscanalsobestainedloose,althoughthesectionsareeasiertohandlewhenmountedonslides. Solutions: Black-GoldIIpowdershouldberesuspendedwithsalinesolution(0.9%NaCl)toafinalconcentrationof0.3%.Simplyaddthe150mgofBlack-GoldIIpowderinto50mlsof0.9%NaClsolutionandmixtoresuspend.OnceresuspendedtheBlack-GoldIIstainingsolutionshouldbestoredinthedarkat4°Candisgoodforupto3months. Staining: 1)Formalin-fixed,non-embeddedtissuesectionsarefirstmountedfromdistilledwaterontogelatin-coatedslidesandthenairdriedat50-60ºCforatleast½houronaslidewarmer. 2)TissuesectionsarethenrehydratedindistilledMilli-Q®waterforabout2minutes. 3)Theslidesarethentransferredtoa0.3%Black-GoldIIsolutiondissolvedin0.9%salinevehicleandheatedto60-65ºC.Afterabout12minutes,microscopicmonitoringoftheextentofthelabelingisrecommended.Thismonitoringshouldberepeatedevery2-3minutesuntilthedesireddegreeofmyelinimpregnationisobserved(seebelow). 4)Rinsetheslidesforabout2minindistilledMilli-Qwater. 5)Transfertheslidestoa1%sodiumthiosulfatesolutionfor3minutes. 6)Rinsetheslideswitheitherthree,fiveminchangesoftapwateror15minsofrunningtapwater. Post-stainingsteps: 7)Dehydratesectionseitherviagraduatedalcoholicsolutionsorbyairdryingonaslidewarmer. 8)Immersesectionsfor1-2mininxyleneorhistoclearandthencoverslipwithanon-polarmountingmedia.

Applications

KeyApplications

Immunohistochemistry

ApplicationNotes

TissueFixation:
Tissuecanbefixedwitheitherformalinor4%PFA.Typicallyanimalsareperfusedwithneutralphosphatebuffered10%formalin(or4%formaldehyde)viatheascendingaorta.Thebrainsarethenremovedandfixedovernightinthesamefixativesolution.Longerfixationtimesaregenerallynotrecommended.Forcryosections,thebrainsmaybeprotectedthroughtreatmentwithsucrosesolution(20-30percent).Subsequentlyfrozenbraintissueiscutonafreezingslidingmicrotome.Note:Black-GoldIIwillnotstainparaffinembeddedorunfixedtissue.

Sectioning:
Eitherfrozenorfixed,non-frozenvibratomesectionscanbeusedandshouldbecutatathicknessof20-40μm.Thesectionsarethentypicallymountedonpositivelychargedslides.Thesectionscanalsobestainedloose,althoughthesectionsareeasiertohandlewhenmountedonslides.

Solutions:
Black-GoldIIpowdershouldberesuspendedwithsalinesolution(0.9%NaCl)toafinalconcentrationof0.3%.Simplyaddthe150mgofBlack-GoldIIpowderinto50mlsof0.9%NaClsolutionandmixtoresuspend.OnceresuspendedtheBlack-GoldIIstainingsolutionshouldbestoredinthedarkat4°Candisgoodforupto3months.

Staining:
1)Formalin-fixed,non-embeddedtissuesectionsarefirstmountedfromdistilledwaterontogelatin-coatedslidesandthenairdriedat50-60ºCforatleast½houronaslidewarmer.
2)TissuesectionsarethenrehydratedindistilledMilli-Q®waterforabout2minutes.
3)Theslidesarethentransferredtoa0.3%Black-GoldIIsolutiondissolvedin0.9%salinevehicleandheatedto60-65ºC.Afterabout12minutes,microscopicmonitoringoftheextentofthelabelingisrecommended.Thismonitoringshouldberepeatedevery2-3minutesuntilthedesireddegreeofmyelinimpregnationisobserved(seebelow).
4)Rinsetheslidesforabout2minindistilledMilli-Qwater.
5)Transfertheslidestoa1%sodiumthiosulfatesolutionfor3minutes.
6)Rinsetheslideswitheitherthree,fiveminchangesoftapwateror15minsofrunningtapwater.

Post-stainingsteps:
7)Dehydratesectionseitherviagraduatedalcoholicsolutionsorbyairdryingonaslidewarmer.
8)Immersesectionsfor1-2mininxyleneorhistoclearandthencoverslipwithanon-polarmountingmedia.

BIOLOGicalInformation

PhysicochemicalInformation

Dimensions

MaterialsInformation

MaterialsInformation

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Millipore/AG400 | BLACK-GOLD® II Stain/AG400/150 mg

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