Chickenswereimmunizedwithactinpurifiedfromrabbitmuscle.Afterrepeatedinjections,immuneeggswerecollected,andtheIgYfractionswerepurifiedfromtheyolks.TheseIgYfractionswerethenaffinity-purifiedusingapeptidecolumn.Thefinalproductcontains50%glycerol(v/v),purifiedantiactinIgYfraction(10mg/ml)spikedwith25ug/mlofaffinitypurifiedanti-actinIgY,andthenfilter-sterilized.