BackgroundInformation | Antibodiestoneuronalproteinshavebecomecriticaltoolsforidentifyingneuronsanddiscerningmorphologicalcharacteristicsincultureandcomplextissue.WhilethelabelingfromclassichistologicaltechniquessuchasGolgistainingandmodernmolecularapproachessuchasGFPconstructsyieldexcellentcytoarchitecturaldetail,theseapproachesaretechnicallychallengingandimpracticalformanyneuroscienceresearchneeds.Neuron-specificantibodiesareconvenientprecisiontoolsusefulinrevealingcytoarchitecture,butarelimitedtotheproteintargetdistributionwithintheneuron,whichmaydiffergreatlyfromnucleustosomatodendriteandaxon.ToachieveascompleteamorphologicalstainingaspossIBLeacrossallpartsofneurons,Milliporehasdevelopedapolyclonalantibodyblendthatreactsagainstkeysomatic,nuclear,dendritic,andaxonalproteinsdistributedacrossthepan-neuronalarchitecturethatcanthenbedetectedbyasinglesecondaryantibody.Thisantibodycocktailhasbeenvalidatedindiversemethods,cellcultureandimmuno-histochemistry,givingresearchersaconvenientandspecificqualitativeandquantitativetoolforstudyingneuronalmorphology. |