Immunogen | Histidine-taggedrecombinantproteincorrespondingtohumanRAD51. |
Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. |
Host | Rabbit |
SpeciesReactivity | |
SpeciesReactivityNote | DemonstratedtoreactwithHuman,Mouse,andRat.Otherhomologies:Bovine,Equine,andCanine(99%sequencehomology). |
AntibodyType | PolyclonalAntibody |
EntrezGeneNumber | |
EntrezGeneSummary | TheproteinencodedbythisgeneisamemberoftheRAD51proteinfamily.RAD51familymembersarehighlysimilartobacterialRecAandSaccharomycescerevisiaeRad51,andareknowntobeinvolvedinthehomologousrecombinationandrepairofDNA.ThisproteincaninteractwiththessDNA-bindingproteinRPAandRAD52,anditisthoughttoplayrolesinhomologouspairingandstrandtransferofDNA.ThisproteinisalsofoundtointeractwithBRCA1andBRCA2,whichmaybeimportantforthecellularresponsetoDNAdamage.BRCA2isshowntoregulateboththeintracellularlocalizationandDNA-bindingABIlityofthisprotein.LossofthesecontrolsfollowingBRCA2inactivationmaybeakeyeventleADIngtogenomicinstabilityandtumOrigenesis.Multipletranscriptvariantsencodingdifferentisoformshavebeenfoundforthisgene.[providedbyRefSeq]. |
GeneSymbol | |
PurificationMethod | AffinityPurfied |
UniProtNumber | |
UniProtSummary | FUNCTION:MayparticipateinacommonDNAdamageresponsepathwayassociatedwiththeactivationofhomologousrecombinationanddouble-strandbreakrepair.BindstosingleanddoublestrandedDNAandexhibitsDNA-dependentATPaseactivity.UnderwindsduplexDNAandformshelicalnucleoproteinfilaments.PlaysaroleinregulatingmitochondrialDNAcopynumberunderconditionsofoxidativestressinthepresenceofRAD51CandXRCC3.
SUBUNITSTRUCTURE:InteractswithBRCA1,BRCA2andeitherdirectlyorindirectlywithp53.InteractswithXRCC3,RAD54LandRAD54B.PartofacomplexwithRAD51CandRAD51B.InteractswithRAD51AP1andRAD51AP2.InteractswithCHEK1/CHK1,andthismayrequirepriorphosphorylationofCHEK1.InteractswiththeMND1-PSMC3IPheterodimer(Bysimilarity).InteractswithOBFC2B.InteractswithRPA1.InteractswithPALB2.InteractswithSWI5/C9orf119,andatlowerlevelwithSFR1/MEIR5.
SUBCELLULARLOCATION:Nucleus.Cytoplasm.Cytoplasm›perinuclearregion.Mitochondrionmatrix.Note:ColocalizeswithRAD51AP1tomultiplenuclearfociuponinductionofDNAdamage.DNAdamageinducesanincreaseinnuclearlevels.
TISSUESPECIFICITY:Highlyexpressedintestisandthymus,followedbysmallintestine,placenta,colon,pancreasandovary.Weaklyexpressedinbreast.
INDUCTION:Stress-inducedincreaseinthemitochondriallevelsisseen.
PTM:Phosphorylated.PhosphorylationofThr-309byCHEK1/CHK1mayenhanceassociationwithchromatinatsitesofDNAdamageandpromoteDNArepairbyhomologousrecombination.
INVOLVEMENTINDISEASE:DefectsinRAD51areacauseofsusceptibilitytobreastcancer(BC)[MIM:114480].Acommonmalignancyoriginatingfrombreastepithelialtissue.Breastneoplasmscanbedistinguishedbytheirhistologicpattern.Invasiveductalcarcinomaisbyfarthemostcommontype.Breastcancerisetiologicallyandgeneticallyheterogeneous.Importantgeneticfactorshavebeenindicatedbyfamilialoccurrenceandbilateralinvolvement.Mutationsatmorethanonelocuscanbeinvolvedindifferentfamiliesoreveninthesamecase.Ref.28
SEQUENCESIMILARITIES:BelongstotheRecAfamily.RAD51subfamily.
Contains1HhHdomain. |
MolecularWeight | ~37kDaobserved.Twoisoforms(isoform1at37kDaandisoform2at26kDa)producedasaresultofalternativesplicingareknowntoexist:. |