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Millipore/AB9876 | Anti-Stargazin Antibody/AB9876/100 µg
  • Millipore/AB9876 | Anti-Stargazin Antibody/AB9876/100 µg

Millipore/AB9876 | Anti-Stargazin Antibody/AB9876/100 µg

价格: ¥4308.00 市场价: 7180.00

货号: AB9876
品牌: Millipore
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    • Description
      CatalogueNumberAB9876
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionAnti-StargazinAntibody
      AlternateNames
      • Stargazin-likeTARP(transmembraneAMPAreceptorregulatoryprotein)
      ProductInformation
      FormatPurified
      PresentationLiquidin130mMTris-Cl,87mMGlycine,pH7.4.
      StorageandShippingInformation
      StorageConditionsMaintainat-80°Cinundilutedaliquotsforupto6monthsafterdateofreceipt.Avoidrepeatedfreezethawcycles.Theantibodymaybekeptat2-8°Cfor1-3weeksafterthawing.
      Applications
      ApplicationThisAnti-StargazinAntibodyisvalidatedforuseinIP,WB,IC,IHforthedetectionofStargazin.
      KeyApplications
      • Immunoprecipitation
      • WesternBlotting
      • Immunocytochemistry
      • Immunohistochemistry
      ApplicationNotesWesternblot:0.1μg/mLonTritonsolublefractionofratbrain.SuggesteddilutionbufferisTBS-Tandblockingbufferis3%skimmilk.Suggestedincubationtimeis3hourstoovernightatroomtemperature.
      Immunohistochemistry:0.25-0.5μg/mLon4%paraformaldehydefixedtissue.SuggesteddilutionbufferisPBS-T.SuggestedpermeABIlizationmethodis0.1%TritonX-100.Suggestedincubationtimeis2hourstoovernightatroomtemperature.
      Immunocytochemistry
      Immunoprecipitation:1-2μg.Suggestedreactionvolumeis200-1000μL.Suggestedtotalproteinconcentrationis2mg/mL.Suggestedincubationtimeis2hoursat2-8°C.SuggestedcaptureagentisProteinA.

      Optimalworkingdilutionsmustbedeterminedbyenduser.
      BIOLOGicalInformation
      ImmunogenGSTfusionproteinfromratstargazin,cytoplasmicdomain.
      HostRabbit
      SpecificityStargazin[Stargazin-likeTARP(transmembraneAMPAreceptorregulatoryprotein)].ByWesternblottheantibodyrecognizesbandsat~35kDaand~55kDaonTritonsolublefractionofratbrain.TheantibodyreactsdominantlywithTARPgamma-8andweaklywithgamma-3andgamma-4.Stargazinisexpressedstronglyinthehippocampusandcerebellum.
      SpeciesReactivity
      • Mouse
      • Rat
      AntibodyTypePolyclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryL-typecalciumchannelsarecomposedoffivesubunits.Theproteinencodedbythisgenerepresentsoneofthesesubunits,gamma,andisoneofseveralgammasubunitproteins.Itisanintegralmembraneproteinthatisthoughttostabilizethecalciumchannelinaninactive(closed)state.Thisproteinissimilartothemousestargazinprotein,mutationsinwhichhavebeenassociatedwithabsenceseizures,alsoknownaspetit-malorspike-waveseizures.ThisgeneisamemberoftheneuronalcalciumchannelgammasubunitgenesubfamilyofthePMP-22/EMP/MP20family.
      GeneSymbol
      • CACNG2
      • stargazin
      • MGC138504
      • MGC138502
      PurificationMethodImmunoAffinityPurified
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:Q9Y698#Thoughttostabilizethecalciumchannelinaninactivated(closed)state.
      SIZE:323aminoacids;35966Da
      SUBUNIT:TheL-typecalciumchanneliscomposedoffivesubunits:alpha-1,alpha-2/delta,betaandgamma.InteractswiththePDZdomainsofDLG4/PSD-95andDLG1/SAP97.MayinteractwithGOPC(Bysimilarity).
      SUBCELLULARLOCATION:Membrane;Multi-passmembraneprotein.
      TISSUESPECIFICITY:Brain.
      PTM:PhosphorylationofThr-321impairsinteractionwithDLG1andDLG4.
      SIMILARITY:SwissProt:Q9Y698##BelongstothePMP-22/EMP/MP20family.CACNGsubfamily.
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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