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Millipore/AB5995 | Anti-Olig3 Antibody/AB5995/100 µg
  • Millipore/AB5995 | Anti-Olig3 Antibody/AB5995/100 µg

Millipore/AB5995 | Anti-Olig3 Antibody/AB5995/100 µg

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货号: AB5995
品牌: Millipore
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    • Description
      CatalogueNumberAB5995
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionAnti-Olig3Antibody
      ProductInformation
      FormatAffinityPurified
      PresentationAffinitypurifiedimmunoglobulin.Precipitatedantibodyinasolutionof50%saturatedammoniumsulfateandPBScontainingnopreservatives.
      StorageandShippingInformation
      StorageConditionsMaintainunopenedvialat-70°Cforupto6months.Avoidrepeatedfreeze/thawcycles.

      PREPARATIONANDUSE:

      Toreconstitutetheantibody,centrifugetheantibodyvialatmoderatespeed(5,000rpm)for5minutestopellettheprecipitatedantibodyproduct.Carefullyremovetheammoniumsulfate/PBSbuffersolutionanddiscard.Itisnotnecessarytoremovealloftheammoniumsulfate/PBSsolution:10μLofresidualammoniumsulfatesolutionwillnotaffecttheresUSPensionoftheantibody.Donotlettheproteinpelletdry,asseverelossofantibodyreactivitycanoccur.

      ResuspendtheantibodypelletinasuitableBIOLOGicalbuffersuchasPBSorTBS(pH7.3-7.5)toafinalconcentrationof1.0mg/mL.Forexample,toachievea1.0mg/mLconcentrationwith50μgofprecipitatedantibody,theamountofbufferneededwouldbe50μL.

      Carefullyaddtheliquidbuffertothepellet.DONOTVORTEX.Mixbygentlestirringwithawidepipettiporgentlefinger-tapping.Lettheprecipitatedantibodyrehydratefor1hourat4-25°Cpriortouse.Smallparticlesofprecipitatedantibodythatfailtoresuspendarenormal.Vialsareoverfilledtocompensateforanylosses.

      Therehydratedantibodysolutionscanbestoredundilutedat2-8°Cfor2monthswithoutanysignificantlossofactivity.Note,thesolutionisnotsterile,thuscareshouldbetakenifproductisstoredat2-8°C.

      Forstorageat-20°C,theadditionofanequalvolumeofglycerolcanbeused,however,itisrecommendedthatACSgradeorhigherglycerolbeused,assignificantlossofactivitycanoccuriftheglycerolusedisnotofhighquality.

      Forlong-termstorageat-70°C,itisrecommendedthattherehydratedantibodysolutionbefurtherdiluted1:1witha2%BSA(fractionV,highest-gradeavailable)solutionmadewiththerehydrationbuffer.Theresulting1%BSA/antibodysolutioncanbealiquotedandstoredfrozenat-70°Cforupto6months.Avoidrepeatedfreeze/thawcycles.
      Applications
      ApplicationAnti-Olig3AntibodydetectslevelofOlig3&hasbeenpublished&validatedforuseinWB,IC,IH.
      KeyApplications
      • WesternBlotting
      • Immunocytochemistry
      • Immunohistochemistry
      ApplicationNotesWesternblot:1:200-1:1,000usingECL.

      Immunohistochemistry:1:200-1:1,000.

      Immunocytochemistry:1:200-1:1,000.

      Optimalworkingdilutionsmustbedeterminedbytheenduser.
      BiologicalInformation
      ImmunogenSyntheticpeptide,aminoacids53-66ofOlig3.
      HostRabbit
      SpecificityRecognizesOlig3.Themolecularweightoftheproteinis27,909Daltons.
      SpeciesReactivity
      • Human
      • Mouse
      AntibodyTypePolyclonalAntibody
      EntrezGeneNumber
      GeneSymbol
      • OLIG3
      • BHLHB7
      • bHLHB7
      • Oligo3
      • Bhlhb7
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:Q7RTU3#MaydeterminethedistinctspecificationprogramofclassAneuronsinthedorsalpartofthespinalcordandsuppressspecificationofclassBneurons.
      SIZE:272aminoacids;29358Da
      SUBCELLULARLOCATION:Nucleus(Bysimilarity).
      SIMILARITY:SwissProt:Q7RTU3##Contains1basichelix-loop-helix(bHLH)domain.
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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