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Millipore/AB5220 | Anti-Growth Associated Protein-43 (GAP-43) Antibody/AB5220/100 µL
  • Millipore/AB5220 | Anti-Growth Associated Protein-43 (GAP-43) Antibody/AB5220/100 µL

Millipore/AB5220 | Anti-Growth Associated Protein-43 (GAP-43) Antibody/AB5220/100 µL

价格: ¥4680.00 市场价: 7800.00

货号: AB5220
品牌: Millipore
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    • Description
      CatalogueNumberAB5220
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionAnti-GrowthAssociatedProtein-43(GAP-43)Antibody
      AlternateNames
      • Neuromodulin
      BackgroundInformationGAP-43,acommonMarkerofdifferentiatingneurons,isexpressedatelevatedlevelsbydevelopingorregeneratingneuronsduringaxongrowth.WhileGAP-43isanintegralmembraneproteinassociatedwiththecytoplasmicsurfaceofaxonalgrowthcones(andsynapses),itisabsentfromdendriticgrowthcones.Inadultbrain,GAP-43isfoundinhighconcentrationinpresynapticareaswherememoryformationisthoughttooccur,suchasfrontalcortex,limbicsystemandhippocampus.PhosphorylationofGAP-43byPKC(atSer41onhuman,mouse,rat,andbovineGAP-43orSer42onchickenandXenopusGAP-43)isspecificallycorrelatedwithcertainformsofsynapticplasticity.
      ProductInformation
      FormatPurified
      Control
      • Ratdorsalrootgangliontissuethathasbeensubjectedtoaspinalnerveligation,culturedneurons.
      PresentationPurifiedrabbitpolyclonalinbuffercontainingPBSwith0.01%sodiumazideasapreservative.
      StorageandShippingInformation
      StorageConditionsStableforupto6monthsat-20ºCinundilutedaliquotsfromdateofreceipt.
      Avoidrepeatedfreeze/thawcycles.Duringshipment,smallvolumesofproductwilloccasionallybecomeentrappedinthesealoftheproductvial.Forproductswithvolumesof200µLorless,werecommendgentlytappingthevialonahardsurfaceorbrieflycentrifugingthevialinatabletopcentrifugetodislodgeanyliquidinthecontainer’scap.
      Applications
      ApplicationAnti-GrowthAssociatedProtein-43(GAP-43)AntibodyisanantibodyagainstGrowthAssociatedProtein-43(GAP-43)foruseinELISA,IC,IH,IP&WB.
      KeyApplications
      • ELISA
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunoprecipitation
      • WesternBlotting
      ApplicationNotesImmunohistochemistry:
      1:1,000dilutionofapreviouslotwasusedon4%paraformaldehyde/PBSfixedor3%Paraformaldehyde/0.1%Gluteraldehyde/PBSfixedtissuesections.

      Immunocytochemistry:
      1:1,000dilutionofapreviouslotwasusedonHippocampalneuronsincultureandPC12cellsstimulatedwithNGF.

      Immunoprecipitation:
      1:100dilutionofapreviouslotwasused.

      ELISA:
      1:2,000dilutionofapreviouslotwasused.
      BIOLOGicalInformation
      ImmunogenRecombinantratGAP-43(completesequence).
      ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
      HostRabbit
      SpecificityRecognizesGrowthAssociatedProtein-43(GAP-43).
      SpeciesReactivity
      • Human
      • Mouse
      • Rat
      • Chicken
      • Bovine
      SpeciesReactivityNoteReactstoahighdegreewithmouse.Reactstoalesserdegreewithrat,human,bovineandchicken.Otherspecieshavenotbeentested.
      AntibodyTypePolyclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryTheproteinencodedbythisgenehasbeentermeda"growth"or"plasticity"proteinbecauseitisexpressedathighlevelsinneuronalgrowthconesduringdevelopmentandaxonalregeneration.Thisencodedproteinisconsideredacrucialcomponentofaneffectiveregenerativeresponseinthenervoussystem.
      GeneSymbol
      • GAP43
      • PP46
      • B-50
      PurificationMethodAmmoniumSulfatePrecipitation
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P17677#Thisproteinisassociatedwithnervegrowth.Itisamajorcomponentofthemotilegrowthconesthatformthetipsofelongatingaxons.
      SIZE:238aminoacids;24803Da
      SUBUNIT:BindscalmodulinwithagreateraffinityintheabsenceofCa(2+)thaninitspresence(Bysimilarity).
      SUBCELLULARLOCATION:Cellmembrane;Peripheralmembraneprotein;Cytoplasmicside.Cellprojection,growthconemembrane;Peripheralmembraneprotein;Cytoplasmicside.Celljunction,synapse.Note=Cytoplasmicsurfaceofgrowthconeandsynapticplasmamembranes.
      PTM:PhosphorylationofthisproteinbyaproteinkinaseCisspecificallycorrelatedwithcertainformsofsynapticplasticity.
      SIMILARITY:SwissProt:P17677##Belongstotheneuromodulinfamily.&Contains1IQdomain.
      MolecularWeight23.6kDa
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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