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Millipore/AB1955 | Anti-VE-Cadherin Antibody, phospho-specific [Tyr658]/AB1955/100 µL
  • Millipore/AB1955 | Anti-VE-Cadherin Antibody, phospho-specific [Tyr658]/AB1955/100 µL

Millipore/AB1955 | Anti-VE-Cadherin Antibody, phospho-specific [Tyr658]/AB1955/100 µL

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货号: AB1955
品牌: Millipore
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    • Description
      CatalogueNumberAB1955
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionAnti-VE-CadherinAntibody,phospho-specific[Tyr658]
      BackgroundInformationVE-cadherinisanendothelialspecificadhesionmoleculethatisessentialforthemaintenanceofendothelialbarrierfunctionandangiogenesis.ItislinkedtotheactinCytoskeletonthroughanumberofadaptorproteinsincludingalpha,beta,andgammacatenin.CytoskeletaldynamicsandphosphorylationregulateVE-cadherinmediatedcellcelladhesion.Theinteractionbetweencytosolicc-terminalSrckinase,SH2domainandphosphorylatedtyrosine658siteofVE-Cadherinregulatesdensitydependentcellproliferation.Phosphorylationoftyrosine658hasbeenshowntoleadtouncouplingofp120-cateninfromthecytoplasmictailofVE-cadherin.Thisphosphorylationeventissufficienttomaintaincellsinamesenchymalstateduringthecellinvasionphaseofangiogenesis.
      ProductInformation
      FormatAffinityPurified
      PresentationAntibodyispurifiedfromrabbitserum.Itiscross-adsorbedtononphospho-peptidecorrespondingtothesiteofphosphorylationthenaffinitypurifiedusingphosphorylatedVEcadherin(Tyr658)peptide.Antibodyissuppliedin100µlphosphate-bufferedsaline(noCa2+,noMg2+),pH7.3,50%glycerol,containing1mg/mlBSA,and0.05%sodiumazide.
      StorageandShippingInformation
      StorageConditionsMaintainat-20°Cforuptooneyearor2°-8°Cforshorttermstoragefromdateofreceipt.
      Avoidrepeatedfreeze/thawcycles.
      Applications
      ApplicationAnti-VE-CadherinAntibody,phospho-specific[Tyr658]detectslevelofVE-Cadherin&hasbeenpublished&validatedforuseinWB.
      KeyApplications
      • WesternBlotting
      BIOLOGicalInformation
      ImmunogenPhosphorylatedVE-cadherin(Tyr658)syntheticpeptidecorrespondingtohumanaminoacidresiduesaroundtyrosine658.Thesequenceisconservedinmouse,rat,porcine,bovine,canine,chickenandchimpanzee.
      HostRabbit
      SpecificityTheantibodydetectsa120kDaproteinofVE-cadherin.
      SpeciesReactivity
      • Human
      SpeciesReactivityNoteThissequenceis100%homologousinmouse,rat,chicken,chimpanzee,cow,dogandpigVE-cadherin.
      AntibodyTypePolyclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryThisgeneisaclassicalcadherinfromthecadherinsuperfamilyandislocatedinasix-cadherinclusterinaregiononthelongarmofchromosome16thatisinvolvedinlossofheterozygosityeventsinbreastandprostatecancer.Theencodedproteinisacalcium-dependentcell-celladhesionglycoproteincomprisedoffiveextracellularcadherinrepeats,atransmembraneregionandahighlyconservedcytoplasmictail.FunctioningasaclassiccadherinbyimpartingtocellstheABIlitytoadhereinahomophilicmanner,theproteinmayplayanimportantroleinendothelialcellbiologythroughcontrolofthecohesionandorganizationoftheintercellularjunctions.Analternativesplicevarianthasbeendescribedbutitsfulllengthsequencehasnotbeendetermined.
      GeneSymbol
      • CDH5
      • VE-cadherin
      • CD144
      • 7B4
      Modifications
      • Phosphorylation
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P33151#Cadherinsarecalciumdependentcelladhesionproteins.Theypreferentiallyinteractwiththemselvesinahomophilicmannerinconnectingcells;cadherinsmaythuscontributetothesortingofheterogeneouscelltypes.Thiscadherinmayplayaimportantroleinendothelialcellbiologythroughcontrolofthecohesionandorganizationoftheintercellularjunctions.Itassociateswithalpha-cateninformingalinktothecytoskeleton.
      SIZE:784aminoacids;87516Da
      SUBCELLULARLOCATION:Celljunction.Cellmembrane;Single-passtypeImembraneprotein(Probable).Note=Foundatcell-cellboundariesandprobablyatcell-matrixboundaries.
      TISSUESPECIFICITY:Endothelialtissuesandbrain.
      SIMILARITY:SwissProt:P33151##Contains5cadherindomains.
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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