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Millipore/AB142 | Anti-Galactocerebroside Antibody/AB142/100 µL
  • Millipore/AB142 | Anti-Galactocerebroside Antibody/AB142/100 µL

Millipore/AB142 | Anti-Galactocerebroside Antibody/AB142/100 µL

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货号: AB142
品牌: Millipore
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    • Description
      Catalogue NumberAB142
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Galactocerebroside Antibody
      Product Information
      FormatSerum
      Control
      • Neonatal cortex
      PresentationRabbit antisera. Liquid. Contains no preservative.
      Storage and Shipping Information
      Storage ConditionsMaintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Applications
      ApplicationAnti-Galactocerebroside Antibody detects level of Galactocerebroside & has been published & validated for use in ELISA, IC, IH.
      Key Applications
      • ELISA
      • Immunocytochemistry
      • Immunohistochemistry
      Applications Not Recommended
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry: suggested starting dilution 1:10-1:50. Not suggested for use on paraffin embedded tissue sections.

      Immunocytochemistry: suggested starting dilution 1:10-1:50. For staining of living cells it is recommended that the antisera be heat inactived prior to using to remove complement. Staining of living cells prior to fixation will show a typical "patching" appearance. Cells fixed prior to staining will have a more solid, "smooth" appearance.

      The diluent of choice is DMEM but other buffers may be used.

      The recommended fixative is 4% paraformaldehyde.

      ELISA: suggested starting dilution 1:50-1:100.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenGalactocerebroside purified from bovine brain.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes Oligodendrocytes in mouse brain culture cells (live). AB142 has also shown staining on fixed mouse brain tissue. Other possible applications are staining of rat and human living brain cells / tissue.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Rabbit
      Antibody TypePolyclonal Antibody
      Gene Symbol
      • GALC
      • galactosylceraminidase
      • galactocerebrosidase
      • Galactosylceramidase
      • GALCERase
      Purification MethodUnpurified
      Physicochemical Information
      Dimensions
      Materials Information
      Materials Information
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