ApplicationNotes | Immunohistochemistryat1:500-1:1,000.
PERFUSIONMIXTUREANDPROTOCOLFORIMMUNOCYTOCHEMICALDETECTIONOFDOPAMINE:Seesuggestedprotocols.
AB122Sshouldbeusedatdilutionsof1:500-1:1,000usingTris-MBScontaining0.5%Tritionand1%normalgoatserum.Theantibodyshouldbeincubated15hoursat+4°Corroomtemperature.Finalworkingdilutionsmustbedeterminedbyenduser.
PostTreatment:
TheimmunocytochemistryprocedureisthencontinuedwiththePAPmethodortheavidin-biotinperoxidasesystem.Note:sinceMBSisknowntoinhibitPAPoravidin-biotinperoxidase,itshouldbeomittedforthisstep.40mMTris-HClwith9%NaCladjustedtopH7.6(usingconcentratedHCl)shouldbeused.
Notations:
MBSisessentialinthepre-washandtheglutaraldehydesolutionsincethisantioxidantprotectsthedopaminemoleculefrombeingreleased.
*Protocolmodifiedfrom:McRae-Degueurce,A.,etal.(1986).BrainResearch376:217-219. |