The antiserum was produced against synthesized peptide derived from human VCP around the phosphorylation site of Ser352.
Immunogen Range
318-367
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:1000
Specificity
VCP (Phospho-Ser352) Antibody detects endogenous levels of VCP only when phosphorylated at Ser352.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Western blot analysis of lysates from NIH/3T3 cells treated with starved 24h, using VCP (Phospho-Ser352) Antibody. The lane on the right is blocked with the phospho peptide.